| Literature DB >> 34192631 |
Takehiko Yamanashi1, Taku Saito2, Tong Yu3, Alexandra Alario3, Katie Comp3, Kaitlyn J Crutchley3, Eleanor J Sullivan3, Zoe-Ella M Anderson3, Pedro S Marra3, Gloria Chang3, Nadia E Wahba3, Sydney S Jellison3, Alissa A Meyer3, Srishti Mathur3, Pratik Pandharipande4, Aihide Yoshino5, Koichi Kaneko6, Sangil Lee7, Hiroyuki Toda5, Masaaki Iwata6, Gen Shinozaki8.
Abstract
It has been suggested that aging and inflammation play key roles in the development of delirium. In the present study, we investigated the differences of the DNAm patterns in the TNF gene between patients with delirium and without. The data and samples derived from previous and ongoing cohort studies were analyzed. DNAm levels of the TNF gene were analyzed using the Illumina EPIC array genome-wide method and pyrosequencing method. Correlations between age and DNAm levels of each CpG were calculated. Several CpG in the TNF gene in blood showed negative correlation between their DNAm and age in delirium cases both with the EPIC array and by the pyrosequencing method. However, there was no CpG that had significant correlation between their DNAm and age regardless of delirium status among buccal samples. On the other hand, among peripheral blood mononuclear cells samples, it was found that several CpG showed negative correlation between their DNAm and age in delirium cases. The evidence of DNAm change in the TNF gene among delirious subjects was demonstrated.Entities:
Keywords: Aging; DNA methylation; Delirium; EPIC array; Pyrosequencing; TNF-alpha
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Year: 2021 PMID： 34192631 DOI： 10.1016/j.neurobiolaging.2021.05.005
Source DB: PubMed Journal: Neurobiol Aging ISSN： 0197-4580 Impact factor: 4.673