Jaime D Martinez1,2,3, Esdras Arrieta2, Andrea Naranjo1,2,4, Pedro Monsalve1,4, Keenan J Mintz5, Jeffrey Peterson2,6, Alejandro Arboleda2, Heather Durkee2,7, Mariela C Aguilar2, Daniel Pelaez7,8, Sander R Dubovy1,4, Darlene Miller1,6, Roger Leblanc5, Guillermo Amescua1,2, Jean-Marie Parel1,2,7. 1. Anne Bates Leach Eye Center, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miller School of Medicine, Miami, FL. 2. Ophthalmic Biophysics Center, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miller School of Medicine, Miami, FL. 3. Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miller School of Medicine, Naples, FL. 4. Florida Lions Ocular Pathology Laboratory, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miller School of Medicine, Miami, FL. 5. Department of Chemistry, University of Miami, Coral Gables, FL. 6. Ocular Microbiology Laboratory, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miami Miller School of Medicine, Miami, FL. 7. Department of Biomedical Engineering, University of Miami, Coral Gables, FL; and. 8. Dr. Al-Rashid Orbital Vision Research Center, Bascom Palmer Eye Institute, Department of Ophthalmology, University of Miller School of Medicine, Miami, FL.
Abstract
PURPOSE: To evaluate the in vivo corneal changes after Rose Bengal photodynamic antimicrobial therapy (RB-PDAT) treatment in New Zealand White rabbits. METHODS: Sixteen rabbits were divided into 5 groups. All groups underwent deepithelialization of an 8 mm diameter area in the central cornea. Group 1: balanced salt solution drops only, group 2: 0.2% RB only, group 3: green light exposure (525 nm, 5.4 J/cm2) only, group 4: 0.1% RB-PDAT, and group 5: 0.1% RB-PDAT. All rabbits were followed clinically. Group 5 rabbits were followed using anterior segment optical coherence tomography (AS-OCT) and clinically. On day 35 after initial treatment, 1 rabbit from group 5 was re-exposed to green light (5.4 J/cm2) to evaluate reactivation of the remaining RB dye, and terminal deoxynucleotyl transferase-mediated UTP-biotin-nick-end labeling assay was performed on corneal cryosections. RESULTS: Complete reepithelization was observed, and corneas remained clear after treatment in all groups. In group 5, AS-OCT revealed a cross-linking demarcation line. AS-OCT showed RB fluorescence and collagen cross-linking in all treated eyes of group 5 animals after 5 weeks of treatment. Photobleached RB retention in the corneal stroma was corroborated by fluorescence confocal microscopy on frozen sections. There was no evidence of a sustained cytotoxic effect through terminal deoxynucleotyl transferase-mediated UTP-biotin-nick-end labeling at 5 weeks. CONCLUSIONS: RB-PDAT with 0.1% RB is a safe procedure. There was no difference clinically and on histopathology compared with control groups. In eyes where RB dye is retained in the corneal stroma after 1 month of treatment, oxidative stress is not evidenced at long term.
PURPOSE: To evaluate the in vivo corneal changes after Rose Bengal photodynamic antimicrobial therapy (RB-PDAT) treatment in New Zealand White rabbits. METHODS: Sixteen rabbits were divided into 5 groups. All groups underwent deepithelialization of an 8 mm diameter area in the central cornea. Group 1: balanced salt solution drops only, group 2: 0.2% RB only, group 3: green light exposure (525 nm, 5.4 J/cm2) only, group 4: 0.1% RB-PDAT, and group 5: 0.1% RB-PDAT. All rabbits were followed clinically. Group 5 rabbits were followed using anterior segment optical coherence tomography (AS-OCT) and clinically. On day 35 after initial treatment, 1 rabbit from group 5 was re-exposed to green light (5.4 J/cm2) to evaluate reactivation of the remaining RB dye, and terminal deoxynucleotyl transferase-mediated UTP-biotin-nick-end labeling assay was performed on corneal cryosections. RESULTS: Complete reepithelization was observed, and corneas remained clear after treatment in all groups. In group 5, AS-OCT revealed a cross-linking demarcation line. AS-OCT showed RB fluorescence and collagen cross-linking in all treated eyes of group 5 animals after 5 weeks of treatment. Photobleached RB retention in the corneal stroma was corroborated by fluorescence confocal microscopy on frozen sections. There was no evidence of a sustained cytotoxic effect through terminal deoxynucleotyl transferase-mediated UTP-biotin-nick-end labeling at 5 weeks. CONCLUSIONS: RB-PDAT with 0.1% RB is a safe procedure. There was no difference clinically and on histopathology compared with control groups. In eyes where RB dye is retained in the corneal stroma after 1 month of treatment, oxidative stress is not evidenced at long term.
Authors: Francisco Halili; Alejandro Arboleda; Heather Durkee; Mukesh Taneja; Darlene Miller; Karam A Alawa; Mariela C Aguilar; Guillermo Amescua; Harry W Flynn; Jean-Marie Parel Journal: Am J Ophthalmol Date: 2016-03-23 Impact factor: 5.258
Authors: Jaime D Martinez; Andrea Naranjo; Guillermo Amescua; Sander R Dubovy; Alejandro Arboleda; Heather Durkee; Mariela C Aguilar; Harry W Flynn; Darlene Miller; Jean-Marie Parel Journal: Cornea Date: 2018-10 Impact factor: 2.651
Authors: Andrea Naranjo; Alejandro Arboleda; Jaime D Martinez; Heather Durkee; Mariela C Aguilar; Nidhi Relhan; Neda Nikpoor; Anat Galor; Sander R Dubovy; Roger Leblanc; Harry W Flynn; Darlene Miller; Jean-Marie Parel; Guillermo Amescua Journal: Am J Ophthalmol Date: 2019-09-05 Impact factor: 5.258
Authors: Penny A Asbell; Christine M Sanfilippo; Christopher M Pillar; Heleen H DeCory; Daniel F Sahm; Timothy W Morris Journal: JAMA Ophthalmol Date: 2015-12 Impact factor: 7.389