Xin-Mei Zhao1, Yuan-Bin Li2, Peng Sun2, Ya-di Pu3, Meng-Jie Shan4,5, Yuan-Meng Zhang6. 1. Ophthalmic Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China. 2. Department of Ophthalmology, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China. 3. Qingdao University, Qingdao, Shandong, China. 4. Department of Plastic Surgery, Peking Union Medical College Hospital, Beijing, China. 5. Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China. 6. Department of Cardiology, The Third Medical Centre of Chinese PLA General Hospital, No. 69, Yongding Road, Hai Dian, Beijing, China.
Abstract
OBJECTIVE: To identify key genes involved in occurrence and development of retinoblastoma. METHODS: The microarray dataset, GSE5222, was downloaded from the gene expression omnibus (GEO) database. Differentially expressed genes (DEGs) between unilateral and bilateral retinoblastoma were identified and functional enrichment analysis performed. The protein-protein interaction (PPI) network was constructed and analysed by STRING and Cytoscape. RESULTS: DEGs were mainly associated with activation of cysteine-type endopeptidase activity involved in apoptotic process and small molecule catabolic process. Seven genes (WAS, GNB3, PTGER1, TACR1, GPR143, NPFF and CDKN2A) were identified as HUB genes. CONCLUSION: Our research provides more understanding of the mechanisms of the disease at a molecular level and may help in the identification of novel biomarkers for retinoblastoma.
OBJECTIVE: To identify key genes involved in occurrence and development of retinoblastoma. METHODS: The microarray dataset, GSE5222, was downloaded from the gene expression omnibus (GEO) database. Differentially expressed genes (DEGs) between unilateral and bilateral retinoblastoma were identified and functional enrichment analysis performed. The protein-protein interaction (PPI) network was constructed and analysed by STRING and Cytoscape. RESULTS: DEGs were mainly associated with activation of cysteine-type endopeptidase activity involved in apoptotic process and small molecule catabolic process. Seven genes (WAS, GNB3, PTGER1, TACR1, GPR143, NPFF and CDKN2A) were identified as HUB genes. CONCLUSION: Our research provides more understanding of the mechanisms of the disease at a molecular level and may help in the identification of novel biomarkers for retinoblastoma.
Authors: Maria-Del-Carmen Silva-Lucero; Jared Rivera-Osorio; Laura Gómez-Virgilio; Gustavo Lopez-Toledo; José Luna-Muñoz; Francisco Montiel-Sosa; Luis O Soto-Rojas; Mar Pacheco-Herrero; Maria-Del-Carmen Cardenas-Aguayo Journal: Diagnostics (Basel) Date: 2022-05-07