Takahito Tamura1,2, Taiki Miyata1,2, Keisuke Hatori3,4, Kazuma Himi1, Takeshi Nakamura1,2, Yurika Toyama1,2, Osamu Takeichi1,4. 1. Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan. 2. Nihon University Graduate School of Dentistry, Dental Research Center, Tokyo, Japan. 3. Department of Endodontics, Nihon University School of Dentistry, Tokyo, Japan; hatori.keisuke@nihon-u.ac.jp. 4. Division of Advanced Dental Treatment, Dental Research Center, Tokyo, Japan.
Abstract
BACKGROUND/AIM: S100A4 expression is associated with the pathology of chronic inflammatory diseases. In this study, we investigated the role of S100A4 and four inflammatory mediators (IL-1β, IκB, IL-10, and TNF-α) in human periapical granulomas (PGs). MATERIALS AND METHODS: S100A4 expression in PGs obtained by apicoectomy was examined by immunohistochemistry. Further, the expression of S100A4 and four inflammatory mediators was compared between PGs and healthy gingival tissues (HGTs) using real-time PCR. RESULTS: In the PGs, S100A4 was found to be expressed in endothelial cells and fibroblasts. Furthermore, real-time PCR revealed that the expression of S100A4 and IL-1β in PGs was significantly higher than that in HGTs. Although a correlation between the expression of S100A4 and IκB or IL-10 was not detected, a positive correlation between the expression of S100A4 and IL-1β or TNF-α was observed. CONCLUSION: The expression of S100A4 correlates with the pathogenesis of PGs.
BACKGROUND/AIM: S100A4 expression is associated with the pathology of chronic inflammatory diseases. In this study, we investigated the role of S100A4 and four inflammatory mediators (IL-1β, IκB, IL-10, and TNF-α) in human periapical granulomas (PGs). MATERIALS AND METHODS: S100A4 expression in PGs obtained by apicoectomy was examined by immunohistochemistry. Further, the expression of S100A4 and four inflammatory mediators was compared between PGs and healthy gingival tissues (HGTs) using real-time PCR. RESULTS: In the PGs, S100A4 was found to be expressed in endothelial cells and fibroblasts. Furthermore, real-time PCR revealed that the expression of S100A4 and IL-1β in PGs was significantly higher than that in HGTs. Although a correlation between the expression of S100A4 and IκB or IL-10 was not detected, a positive correlation between the expression of S100A4 and IL-1β or TNF-α was observed. CONCLUSION: The expression of S100A4 correlates with the pathogenesis of PGs.
Authors: Malin C Erlandsson; Mattias D Svensson; Ing-Marie Jonsson; Li Bian; Noona Ambartsumian; Sofia Andersson; ZhiQi Peng; Jukka Vääräniemi; Claes Ohlsson; Karin M E Andersson; Maria I Bokarewa Journal: Biochim Biophys Acta Date: 2013-07-02
Authors: Lucie Andrés Cerezo; Martina Remáková; Michal Tomčik; Steffen Gay; Michel Neidhart; Eugene Lukanidin; Karel Pavelka; Mariam Grigorian; Jiří Vencovský; Ladislav Šenolt Journal: Rheumatology (Oxford) Date: 2014-03-18 Impact factor: 7.580