Nathaniel F Wu1,2, Justin Wu3,4, Jun Yamamoto1,4, Yusuke Aoki1,4, Chihiro Hozumi5, Michael Bouvet4, Robert M Hoffman6,4. 1. AntiCancer Inc, San Diego, CA, U.S.A. 2. Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, U.S.A. 3. Department of General Surgery, Kaiser Permanente San Diego Medical Center, San Diego, CA, U.S.A. 4. Department of Surgery, University of California, San Diego, CA, U.S.A. 5. AntiCancer Japan Inc, Narita, Japan. 6. AntiCancer Inc, San Diego, CA, U.S.A.; all@anticancer.com.
Abstract
BACKGROUND/AIM: Sarcomas of the breast are extremely rare malignant tumors and comprise only 5% of all sarcomas and fewer than 1% of breast cancers. Primary osteosarcoma of the breast is histologically indistinguishable from osteosarcoma of the bone. Effective therapies of this recalcitrant disease have not yet been developed. MATERIALS AND METHODS: A patient-derived xenograft (PDX) mouse model of primary osteosarcoma of the breast was established by subcutaneous implantation of the surgical specimen, along with surrounding normal tissue. Hematoxylin and eosin (H&E) staining was performed on paraffin-embedded histological sections of the original tumor resected from the patient and from implanted tumors that grew in nude mice. RESULTS: Tumors grew in 46 of 51 mice implanted with the original surgical specimen. The H&E-stained slides of the mouse-grown tumor and the original patient tumor matched, both showing large areas of spindle-shaped cells, characteristic of osteosarcoma. CONCLUSION: The first PDX mouse model of primary breast osteosarcoma was established which will enable testing of novel therapeutics as well as basic research of osteosarcoma of the breast.
BACKGROUND/AIM: Sarcomas of the breast are extremely rare malignant tumors and comprise only 5% of all sarcomas and fewer than 1% of breast cancers. Primary osteosarcoma of the breast is histologically indistinguishable from osteosarcoma of the bone. Effective therapies of this recalcitrant disease have not yet been developed. MATERIALS AND METHODS: A patient-derived xenograft (PDX) mouse model of primary osteosarcoma of the breast was established by subcutaneous implantation of the surgical specimen, along with surrounding normal tissue. Hematoxylin and eosin (H&E) staining was performed on paraffin-embedded histological sections of the original tumor resected from the patient and from implanted tumors that grew in nude mice. RESULTS:Tumors grew in 46 of 51 mice implanted with the original surgical specimen. The H&E-stained slides of the mouse-grown tumor and the original patienttumor matched, both showing large areas of spindle-shaped cells, characteristic of osteosarcoma. CONCLUSION: The first PDX mouse model of primary breast osteosarcoma was established which will enable testing of novel therapeutics as well as basic research of osteosarcoma of the breast.
Authors: Noriyuki Masaki; Yutaka Yonemura; Nathaniel F Wu; Carissa Samonte; Chihiro Hozumi; Yutaro Kubota; Yusuke Aoki; Michael Bouvet; Jun Miyazaki; Robert M Hoffman Journal: In Vivo Date: 2022 Jul-Aug Impact factor: 2.406
Authors: Dimitrios E Magouliotis; Kostas Lafazanis; Fani Koutsougianni; Nikos Sakellaridis; Maria Ioannou; Dimitris Zacharoulis; Konstantinos Dimas Journal: In Vivo Date: 2022 May-Jun Impact factor: 2.406
Authors: Noriyuki Masaki; Nathaniel F Wu; Yusuke Aoki; Jun Yamamoto; Jun Miyazaki; Robert M Hoffman Journal: In Vivo Date: 2021 Nov-Dec Impact factor: 2.155