Literature DB >> 34179266

Transient Gene Expression for the Characteristic Signal Sequences and the Estimation of the Localization of Target Protein in Plant Cell.

Mikhail Berestovoy1,2, Alexander Tyurin1,2, Ksenia Kabardaeva1,2, Yuriy Sidorchuk3, Artem Fomenkov1, Alexander Nosov1, Irina Goldenkova-Pavlova1.   

Abstract

We have proposed and tested a method for characterization of the signal sequences and determinations of target protein localization in a plant cell. This method, called the AgI-PrI, implies extraction of protoplasts from plant tissues after agroinfiltration. The suggested approach combines the advantages of two widely used methods for transient gene expression in plants-agroinfiltration and transfection of isolated protoplasts. The AgI-PrI technic can be applied to other plant species.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Agroinfiltration; Protoplast isolation; Signal sequences; Subcellular localization; Tobacco; Transient expression

Year:  2018        PMID： 34179266      PMCID： PMC8203870          DOI： 10.21769/BioProtoc.2738

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  1 in total

1.  Comparison of several Nicotiana species as hosts for high-scale Agrobacterium-mediated transient expression.

Authors:  Y V Sheludko; Y R Sindarovska; I M Gerasymenko; M A Bannikova; N V Kuchuk
Journal:  Biotechnol Bioeng       Date:  2007-02-15       Impact factor: 4.530
  1 in total
  1 in total

1.  Modulation of the Translation Efficiency of Heterologous mRNA and Target Protein Stability in a Plant System: The Case Study of Interferon-αA.

Authors:  Alexander A Tyurin; Orkhan Mustafaev; Aleksandra V Suhorukova; Olga S Pavlenko; Viktoriia A Fridman; Ilya S Demyanchuk; Irina V Goldenkova-Pavlova
Journal:  Plants (Basel)       Date:  2022-09-20
  1 in total

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