Literature DB >> 34179257

Radioactive Tracer Feeding Experiments and Product Analysis to Determine the Biosynthetic Capability of Comfrey (Symphytum officinale) Leaves for Pyrrolizidine Alkaloids.

Thomas Stegemann1, Lars H Kruse1, Dietrich Ober1.   

Abstract

This protocol delivers a method to determine the biosynthetic capability of comfrey leaves for pyrrolizidine alkaloids independently from other organs like roots or flowers. The protocol applies and combines radioactive tracer experiments with standard and modern techniques like thin layer chromatography (TLC), solid-phase extraction (SPE), high-performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC-MS).
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  Alkaloid; GC-MS; Plant-organ specificity; Radio-HPLC; Radio-TLC; SPE; Tracer

Year:  2018        PMID： 34179257      PMCID： PMC8203869          DOI： 10.21769/BioProtoc.2719

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

1.  The two facies of pyrrolizidine alkaloids: the role of the tertiary amine and its N-oxide in chemical defense of insects with acquired plant alkaloids.

Authors:  R Lindigkeit; A Biller; M Buch; H M Schiebel; M Boppré; T Hartmann
Journal:  Eur J Biochem       Date:  1997-05-01

2.  Identification of a Second Site of Pyrrolizidine Alkaloid Biosynthesis in Comfrey to Boost Plant Defense in Floral Stage.

Authors:  Lars H Kruse; Thomas Stegemann; Christian Sievert; Dietrich Ober
Journal:  Plant Physiol       Date:  2017-03-08       Impact factor: 8.340

3.  Sequestration, metabolism and partial synthesis of tertiary pyrrolizidine alkaloids by the neotropical leaf-beetle Platyphora boucardi.

Authors:  T Hartmann; C Theuring; L Witte; J M Pasteels
Journal:  Insect Biochem Mol Biol       Date:  2001-10       Impact factor: 4.714

4.  Tissue distribution, core biosynthesis and diversification of pyrrolizidine alkaloids of the lycopsamine type in three Boraginaceae species.

Authors:  Cordula Frölich; Dietrich Ober; Thomas Hartmann
Journal:  Phytochemistry       Date:  2007-02-22       Impact factor: 4.072

5.  Distinct cell-specific expression of homospermidine synthase involved in pyrrolizidine alkaloid biosynthesis in three species of the boraginales.

Authors:  Daniel Niemüller; Andreas Reimann; Dietrich Ober
Journal:  Plant Physiol       Date:  2012-05-07       Impact factor: 8.340
  5 in total
  1 in total

1.  Insights into polyamine metabolism: homospermidine is double-oxidized in two discrete steps by a single copper-containing amine oxidase in pyrrolizidine alkaloid biosynthesis.

Authors:  Mahmoud M Zakaria; Thomas Stegemann; Christian Sievert; Lars H Kruse; Elisabeth Kaltenegger; Ulrich Girreser; Serhat S Çiçek; Manfred Nimtz; Dietrich Ober
Journal:  Plant Cell       Date:  2022-05-24       Impact factor: 12.085
  1 in total

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