Literature DB >> 34179251

Determination of DNA Damage in the Retina Photoreceptors of Drosophila.

Damulewicz Milena1, Pyza Elzbieta1.   

Abstract

The retina is sensitive for light damages, because of direct light exposure, especially intense blue and UV light, which increase level of ROS and other toxic phototransduction products in photoreceptor cells. In our previous work ( Damulewicz et al., 2017a and 2017b), we used 8-oxo-deoxyguanosine (8-OHdG) as a marker for oxidative stress to investigate the role of heme oxygenase in DNA protection against UV light. In this protocol, we showed how to determine the level of DNA damages in the retina using immunohistochemical staining.
Copyright © 2018 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  8-Oxo-deoxyguanosine; DNA damage; Oxidative stress; Retina degeneration; UV light

Year:  2018        PMID： 34179251      PMCID： PMC8203978          DOI： 10.21769/BioProtoc.2708

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  15 in total

1.  Benzo[a]pyrene diol-epoxide-I-DNA and oxidative DNA adducts associated with gastric adenocarcinoma.

Authors:  B M Lee; J J Jang; H S Kim
Journal:  Cancer Lett       Date:  1998-03-13       Impact factor: 8.679

2.  Prognostic and aetiological relevance of 8-hydroxyguanosine in human breast carcinogenesis.

Authors:  J Musarrat; J Arezina-Wilson; A A Wani
Journal:  Eur J Cancer       Date:  1996-06       Impact factor: 9.162

3.  Mapping histological levels of 8-hydroxy-2'-deoxyguanosine in female reproductive organs.

Authors:  Luiz Fernando Pina de Carvalho; Mauricio Simões Abrão; Charles Biscotti; Rakesh Sharma; Ashok Agarwal; Tommaso Falcone
Journal:  J Mol Histol       Date:  2012-10-25       Impact factor: 2.611

Review 4.  Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification.

Authors:  Ron Kohen; Abraham Nyska
Journal:  Toxicol Pathol       Date:  2002 Nov-Dec       Impact factor: 1.902

5.  MutT protein specifically hydrolyses a potent mutagenic substrate for DNA synthesis.

Authors:  H Maki; M Sekiguchi
Journal:  Nature       Date:  1992-01-16       Impact factor: 49.962

6.  Haeme oxygenase protects against UV light DNA damages in the retina in clock-dependent manner.

Authors:  Milena Damulewicz; Agnieszka Loboda; Alicja Jozkowicz; Jozef Dulak; Elzbieta Pyza
Journal:  Sci Rep       Date:  2017-07-12       Impact factor: 4.379

7.  Human 8-oxoguanine DNA glycosylase increases resistance to hyperoxic cytotoxicity in lung epithelial cells and involvement with altered MAPK activity.

Authors:  S Kannan; H Pang; D C Foster; Z Rao; M Wu
Journal:  Cell Death Differ       Date:  2006-02       Impact factor: 15.828

Review 8.  Pulmonary oxidative stress, inflammation and cancer: respirable particulate matter, fibrous dusts and ozone as major causes of lung carcinogenesis through reactive oxygen species mechanisms.

Authors:  Athanasios Valavanidis; Thomais Vlachogianni; Konstantinos Fiotakis; Spyridon Loridas
Journal:  Int J Environ Res Public Health       Date:  2013-08-27       Impact factor: 3.390

9.  Simultaneous determination of 8-oxo-2'-deoxyguanosine and 8-oxo-2'-deoxyadenosine in human retinal DNA by liquid chromatography nanoelectrospray-tandem mass spectrometry.

Authors:  Bin Ma; Meng Jing; Peter W Villalta; Rebecca J Kapphahn; Sandra R Montezuma; Deborah A Ferrington; Irina Stepanov
Journal:  Sci Rep       Date:  2016-03-16       Impact factor: 4.379

10.  Interactions Between the Circadian Clock and Heme Oxygenase in the Retina of Drosophila melanogaster.

Authors:  Milena Damulewicz; Agnieszka Loboda; Alicja Jozkowicz; Jozef Dulak; Elzbieta Pyza
Journal:  Mol Neurobiol       Date:  2016-08-13       Impact factor: 5.590
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