| Literature DB >> 34179245 |
Elia Angelino1,2,3, Simone Reano1,2, Michele Ferrara1,2,3, Emanuela Agosti1,2, Hana Sustova1,2, Valeria Malacarne1,3, Sara Clerici3, Andrea Graziani1,2,3, Nicoletta Filigheddu1,2.
Abstract
Satellite cell (SC) transplantation represents a powerful strategy to investigate SC biology during muscle regeneration. We described here a protocol for SC isolation from green fluorescent protein (GFP)-expressing mice and their transplantation into murine muscles. This procedure was originally used to assess the effects of the hormone unacylated ghrelin on muscle regeneration, in particular evaluating how the increase of unacylated ghrelin in the recipient muscle affected the engraftment of donor SCs ( Reano et al., 2017 ).Entities:
Keywords: Cardiotoxin; Engraftment; Muscle excision; Muscle stem cells; Regeneration; Satellite cells; Skeletal muscle; Transplant
Year: 2018 PMID： 34179245 PMCID： PMC8203946 DOI： 10.21769/BioProtoc.2696
Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325