| Literature DB >> 34174416 |
Yasuhiko Nakao1, Pouya Amrollahi2, Gopanandan Parthasarathy3, Amy S Mauer3, Tejasav S Sehrawat3, Patrick Vanderboom4, K Sreekumaran Nair4, Kazuhiko Nakao5, Alina M Allen3, Tony Y Hu6, Harmeet Malhi7.
Abstract
There is increasing interest in the development of minimally invasive biomarkers for the diagnosis and prognosis of NAFLD via extracellular vesicles (EV). Plasma EVs were isolated by differential ultracentrifugation and quantified by nanoparticle tracking analysis from pre (n = 28) and post (n = 28) weight loss patients. In the pre weight loss group 22 had NAFLD. Nanoplasmon enhanced scattering (nPES) of gold nanoparticles conjugated to hepatocyte-specific antibodies was employed to identify hepatocyte-specific EVs. Complex lipid panel and targeted sphingolipids were performed. Logistic regression analysis was used to identify predictors of NAFLD. Plasma levels of EVs and hepatocyte-derived EVs are dynamic and decrease following NAFLD resolution due to weight loss surgery. Hepatocyte-derived EVs correlate with steatosis in NAFLD patients and steatosis and inflammation in NASH patients. Plasma levels of small EVs correlate with EV sphingolipids in patients with NASH. Hepatocyte-derived EVs measured by the nPES assay could serve as a point-of-care test for NAFLD.Entities:
Keywords: Bariatric surgery; Exosome; Fatty liver; Sphingolipid
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Year: 2021 PMID： 34174416 PMCID： PMC8418232 DOI： 10.1016/j.nano.2021.102430
Source DB: PubMed Journal: Nanomedicine ISSN： 1549-9634 Impact factor: 6.096