| Literature DB >> 34172588 |
Nicholas A Gherardin1,2, Samuel J Redmond3,2, Hamish E G McWilliam3,4, Catarina F Almeida3,2, Katherine H A Gourley3,2, Rebecca Seneviratna3,2, Shihan Li3,2, Robert De Rose3,2, Fiona J Ross3,2, Catriona V Nguyen-Robertson3,2, Shian Su5,6, Matthew E Ritchie5,6, Jose A Villadangos3,4, D Branch Moody7, Daniel G Pellicci3,2,8, Adam P Uldrich3,2, Dale I Godfrey1,2.
Abstract
CD1c presents lipid-based antigens to CD1c-restricted T cells, which are thought to be a major component of the human T cell pool. However, the study of CD1c-restricted T cells is hampered by the presence of an abundantly expressed, non-T cell receptor (TCR) ligand for CD1c on blood cells, confounding analysis of TCR-mediated CD1c tetramer staining. Here, we identified the CD36 family (CD36, SR-B1, and LIMP-2) as ligands for CD1c, CD1b, and CD1d proteins and showed that CD36 is the receptor responsible for non-TCR-mediated CD1c tetramer staining of blood cells. Moreover, CD36 blockade clarified tetramer-based identification of CD1c-restricted T cells and improved identification of CD1b- and CD1d-restricted T cells. We used this technique to characterize CD1c-restricted T cells ex vivo and showed diverse phenotypic features, TCR repertoire, and antigen-specific subsets. Accordingly, this work will enable further studies into the biology of CD1 and human CD1-restricted T cells.Entities:
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Year: 2021 PMID： 34172588 PMCID： PMC8418821 DOI： 10.1126/sciimmunol.abg4176
Source DB: PubMed Journal: Sci Immunol ISSN： 2470-9468