Physiological Exercise and Post-Exercise Effects of Inverse Sequences of Combined Bench-Step Aerobics and Resistance Exercise.

The purpose of this study was to compare the effects of using inverse sequences of combined bench-step aerobics and resistance exercise on cardiorespiratory, hemodynamic and perceptual variables during exercise and one-hour post-exercise. The tested sequences were bench-step aerobics immediately before and immediately after resistance exercise. Thirteen apparently healthy and physically active women (age = 21.9 ± 6.1 years, body height = 160 ± 0.1 cm, body mass = 58.8 ± 7.5 kg, estimated fat mass = 17.2 ± 2.0% and estimated maximal oxygen uptake = 37.5 ± 2.6 mL∙kg-1∙min-1) performed a 20-minute bench-step aerobics routine, immediately before (STEP_RES) and after (RES_STEP) resistance exercise. Oxygen uptake and heart rate were continuously measured, systolic and diastolic blood pressure, body temperature and perceived exertion were measured at rest, immediately after each type of exercise and at 15, 30, 45 and 60 min after exercise. When resistance exercise was performed first (RES_STEP), oxygen uptake was higher (23.2 ± 3.9 vs. 20.5 ± 4.8 mL∙kg-1∙min-1), but the heart rate (164.5 ± 9.1 vs. 173.9 ± 11.7 bpm) and body temperature (36.5 ± 0.4 vs. 37.6 ± 0.6 ºC) were lower. In both sequences, the type of exercise performed first was pointed out with a lower perceived exertion or lower perceived intensity. Exercise and 60-min post-exercise blood pressure had a similar response in both sequences, and systolic blood pressure along with diastolic blood pressure were lower than pre-exercise from 30 min until 60 min post-exercise. The results suggest that the sequence affected oxygen uptake and perceived exertion during exercise and that this total workload, despite the sequence, promoted a post-exercise blood pressure decrease in normotensive participants.

Introduction

Physically active people often perform aerobic and resistance exercises within the same training session in time-saving strategy to benefit from specific adaptations of both types of exercise. Some studies suggest that combining these two types of exercise can improve long-term cardiorespiratory fitness and musculoskeletal function (Izquierdo et al., 2004; Kang et al., 2009; Kraemer et al., 2001), but others found that such combinations result in lower magnitude responses when compared to each type of exercise being performed separately (Kraemer et al., 1995; Larose et al., 2012; Nelson et al., 1990; Schumann et al., 2015).

The acute physiological effects of isolated or combined exercise (aerobic and resistance), in general, are increases in the heart rate (HR), energy expenditure, oxygen uptake (VO2), blood pressure (BP), metabolic rate and related thermic effect. When combined in the same exercise session, resistance exercise performed before aerobic exercise has been related with a higher lipolysis rate and total exercise energy expenditure (Kang et al., 2009; Goto et al., 2005). In contrast, when resistance exercise is performed after aerobic exercise there seems to be an increase in post-exercise oxygen uptake (Drummond et al., 2005). Additionally, there is a higher HR, perceived exertion (RPE) and VO2 during aerobic exercise when it is performed after resistance exercise (Drummond et al., 2005). The BP increases during exercise and decreases afterwards, often to values below those measured pre-exercise (Keese et al., 2011; MacDonald, 2002; MacDonald et al., 1999; Rezk et al., 2006). This repeated post-exercise BP effect has been identified as an important non-pharmacologic strategy for the prevention of hypertension and other lifestyle diseases (Pescatello et al., 2004), occurring with higher magnitude after more intense cardiovascular exercise (Forjaz et al., 2004; Pescatello et al., 2004) and in low- to moderate-intensity resistance exercise (Rezk et al., 2006).

The post-exercise BP effect of combined aerobic and resistance exercise seems similar to isolated aerobic exercise, and is more effective than isolated resistance exercise; however, the available studies present variated methodologies that often compare different exercise doses and, also, methodologies that do not identify the effects of inversing the sequence. Despite all, it seems clear that previous intensity and the type of exercise play an important role in physiological variables related to metabolic, hemodynamic and thermoregulatory specific responses.

Therefore, the purpose of the present study was to compare the effects of using inverse sequences of combined bench-step aerobics and resistance exercise on cardiorespiratory, hemodynamic and perceptual variables during exercise and one-hour post-exercise. The tested sequences were bench-step aerobics immediately before (STEP_RES) and immediately after (RES_STEP) two sets of low-weight high-volume resistance exercises. We hypothesized that despite the similarity of total combined exercise load of bench-step aerobics exercise and low-weight high-volume resistance exercise, changing the sequence would affect the exercise and post-exercise physiological responses.

Methods

Participants

The sample comprised 13 female volunteers, university students, who met the inclusion criteria of being physically active (Pollock et al., 1998), apparently healthy (reporting no metabolic, cardiovascular or respiratory diseases, neither cardiovascular risk factors), taking no medications and had regular menstrual cycles (controlled during the 6 months before data collection) (Table 1). Participants were familiarized with bench-step aerobics and resistance exercises, due to their previous experience from the Sport Sciences bachelor curriculum. All participants were informed of the study procedures and gave their written positive consent. The procedures followed the Declaration of Helsinki and a national institution authority review board granted ethical approval (PEst-OE/SAU/UI4045/2013).

Table 1

Physical and physiological characteristics of participants (N = 13), the values are presented as mean and standard deviations (SD) and intra-class correlation coefficients (ICC).

Variables	Mean ± SD
Age (years)	21.9 ± 6.1
Height (cm)	160.6 ± 0.1
Body mass (kg)	58.8 ± 7.5
Fat mass (%)	17.2 ± 2.0
VO2max, predicted (mL∙kg-1∙min-1)	37.5 ± 2.6
Systolic Blood Pressure (mmHg)	114.0 ± 9.6
Diastolic Blood Pressure (mmHg)	69.5 ± 8.6
HRmax, predicted (bpm)	204.1 ± 6.1
1-RM free weight test
Bent-over Row (kg)	12.5 ± 2.5 (ICC=0.97)
Squat shoulder width (kg)	50.7 ± 0.2 (ICC=0.98)
Biceps Curl shoulder width (kg)	8.6 ± 2.06 (ICC=0.98)
Squat beyond shoulders width (kg)	50.7 ± 0.2 (ICC=0.98)
Deltoid shoulder width (kg)	6.8 ± 1.3 (ICC=0.95)

For the protocol preparation, participants were asked not to ingest alcohol or caffeine and to refrain from exercise during the 48 hours before the experiment. They were advised to drink 33 mL∙kg-1 body-weight of water during the 24 hours before the experiment and 5–7 mL∙kg-1 bodyweight during the three hours preceding the data collection. They completed a 1-day food diary on the day before the first experimental trial and repeated the diary before the second trial.

Measures

Maximum-strength was measured to adjust the exercise load, using the 1-RM method as described earlier by Kraemer and Fry (1995). The re-test was performed after one week and showed high intra-class correlation coefficients (Table 1). To predict maximal aerobic power (VO2max), the Queens-College Step-Test was used accordingly to instructions previously described (Mcardle et al., 1972) and VO2max was derived from the equation (1): VO2max (ml∙kg-1∙min-1) = 65.81 − (0.1847∙HR).

VO2 and the HR were measured using a gas analyzer (COSMED® K4b2, Rome, Italy) with a transmitter band (T61 Polar®, Kempele, Finland). Breath-by-breath and beat-by-beat data were smoothed to one-min average. After 15 min in a sitting position, the average HR and gases of the last 10 min were used for pre-exercise data. For the exercise data, the average values of each 20 min bench-step and 10 min of resistance exercise were used. The average values of the first 15 min after exercise were used for VO2 post-exercise data. The system was calibrated according to the manufacturer's instructions (COSMED® K4b2, Rome, Italy).

The BP was measured using an aneroid sphygmomanometer (Artsana, Standard, Italy). In the last minute of the pre-exercise period and during recovery (at the 14th, 29th, 44th and 59th min), the measurements were performed with participants seated quietly. To measure the BP, immediately after the bench-step and each resistance exercise set (the average value was used), the measurements were performed in a standing position to avoid the postural variation effect on BP. All measurements were done by the same technician and according to standardized instructions (Netea et al., 2003).

The exercise rate pressure product, that is considered a relevant indirect marker of myocardial requirements, was calculated for each type of exercise (i.e. bench-step aerobics and resistance exercises) according to the equation (2): RPP = HR ∙ SBP / 1000.

Body temperature was measured with an auricular thermometer (ThermoScan Plus IRT 3520, Braun) during the 10th and 20th min for the bench-step exercise routine and after each resistance exercise set, in the 14th, 29th, 44th and 59th min of recovery, and two measurements were taken to calculate the arithmetic mean.

The physiologic strain index (a physiological marker that combines cardiovascular and thermoregulatory responses) was calculated for each type of exercise according to Moran et al. (1998) equation (3): PSI= [5 (TtyT - Ttyo) ∙ (39.5 - Ttyo) – 1 + 5 (HRT - HRo) ∙ (180 - HRo) - 1] where Ttyo and HRo were the baseline aural canal temperature and heart rate measurements and TtyT and HRT were the endpoint aural canal temperature and heart rate measurements.

The RPE was measured using the 6-20 Borg scale at minutes 10 and 20 for the bench-step exercise routine and after each resistance exercise set according to normalized instructions (Borg, 1985) and the average was used to establish the exercise values.

Design and Procedures

Each participant went to the laboratory on seven occasions comprehending: study clarification, characterization and maximal aerobic power testing, familiarization with the resistance exercises and one-repetition maximum (1-RM) testing and two experimental trials. The two trials were randomly assigned, started at the same hour, and were separated by 72 hours to seven days to ensure complete recovery and were performed during the follicular phase of the menstrual cycle.

The low-weight high-volume resistance protocol consisted of a circuit of two sets of five exercises at 50% 1-RM with the same 1-RM test order: (a) bent-over row; (b) squat shoulder width; (c) biceps curl; (d) squat beyond shoulder width; and (e) deltoid (shoulder lateral abduction). The resistance exercises were performed with free weights and supervised to ensure adequate technique, intensity and cadence (2.0 s for the concentric and 2.0 s for the eccentric phase) and to perform the repetitions within a full range of motion (until failure). The rest interval between sets and exercises was very short and allowed only to change the weight and body position.

The bench-step aerobics routine consisted of 20 min of uninterrupted steady-state exercise performed on a 20 cm bench-step platform (Reebok ®), with music at a cadence of 132 bpm (Table 2). The exercise was performed individually, although it is not the common method for bench-step aerobics, and the exercise target zone was set between 70-89% of heart rate reserve (HRR) according to consensual guidelines (Garber et al., 2011). Participants were verbally encouraged to maintain appropriate technical movements.

Table 2

Bench-step aerobics exercise warm-up and choreography format with first and second 32 counts routines.

Basic bench‐step movements	Leader foot	Repetitions	Counts	Arm movements
Warm up
Alternated knee	Right/Left	4	16	Lateral pull down (8 times)
Basic step	Right	2	8	Front pull down (4 times)
Three knee repeaters	Right	1	8	90 degrees arms butterfly (3 times)
Start with left foot
1st routine
Basic step with propulsion	Right	2	8	Alternated biceps curl (4 times)
Alternated knee	Right/Left	2	8	Lateral pull down (4 times)
V step	Right	2	8	Alternated overhead arm elevation (2 times)
Three knee repeaters	Right	1	8	90 degrees arms butterfly (3 times)
Start with left foot
2nd routine
Basic step with backward	Right	1	8	Elevated arms in front at shoulders line (2
lunge				times)
Leg flexion	Right/Left	2	8	Upright row arms
Backward leg extension	Right/Left	2	8	Arms forward fully extended
Three alternated knee repeaters	Right	1	8	Alternated 90 degrees arms butterfly (3 times)
Start with left foot

Testing day procedures

On the experimental days, participants arrived at the laboratory 30 min before beginning the exercise protocol, and remained seated while the HR monitor and the gas analyzer vest were fixed (Hans Rudolph, Kansas City, USA). After these procedures, they were asked to remain seated and inactive in a chair for 15 minutes (pre-exercise). After the pre-exercise period, participants performed a two-min standardized step warm-up (Table 2), followed by one of the STEP_RES or RES_STEP sequences.

From the beginning of the pre-exercise period, VO2, VCO2 and VE and HR were continuously measured during the exercise trial and through 15 minutes of recovery, with the HR being measured until the end of recovery. The systolic blood pressure (SBP), diastolic blood pressure (DBP) and body temperature were measured in the last minute of the pre-exercise period, immediately after the end of the step routine, and after each resistance exercise set. Immediately after, participants remained seated and quiet for 60 min (divided into four periods of 15 min of recovery), during which BP and body temperature were measured in the last minute of each 15 min period. The RPE was measured in the middle and at the end of the bench-step exercise routine and after each set of resistance exercise.

The environment temperature (23-25 ºC), atmospheric pressure (712-720 mmHg) and relative humidity (45-50 %) were controlled during all trials. Participants used the same sportswear and sneakers in both experimental trials.

Statistical analysis

All data were inspected using box-and-whiskers plots to identify outliers and data entry errors. The distributions were tested using the Shapiro-Wilk test. The paired sample student t-test was used to ensure similar pre-exercise conditions in all variables, and also to test for differences between STEP_RES and RES_STEP sequences, during bench-step and resistance exercises. The Wilcoxon test was used when non-normal distributions were identified. Repeated measures ANOVA (2 × 5) was used to test the post-exercise effect of the session sequence (STEP_RES and RES_STEP) in five periods (pre-exercise and post-exercise split into 4 periods) and was followed by a posthoc comparison to identify differences between periods. Cohen's d effect sizes and eta square values (η2) were applied to identify the magnitude of differences. All analyses were carried out using the SPSS (Version 16.0, Chicago, IL) and an alpha level of 5%.

Results

There were no differences between pre-exercise values of VO2, SBP, DBP, HR, and body temperature, confirming identical conditions for both experimental trial sequences (STEP_RES and RES_STEP). During the bench-step exercise, the participants’ HRR percentage was 81% in STEP_RES and 82% in RES_STEP. During the resistance exercise, a higher volume (weight × repetition × set) was performed during the RES_STEP sequence than in the STEP_RES sequence for all resistance exercises. However, there were differences only in the biceps curl (p = 0.003).

No differences were found in SBP, DBP and RPP responses between STEP_RES and RES_STEP sequences in any studied period during exercise and post-exercise periods. However, the session sequence promoted a singular effect on VO2, HR, RPE, body temperature and PSI response during the exercise (Table 3).

Table 3

Blood pressure, cardiovascular, respiratory and perceptual responses during bench-step aerobics and resistance exercise periods for the inverse session sequence (STEP_RES and RES_STEP).

	STEP_RES		RES_STEP
Variables	Bench‐Step	Resistance	Resistance	Bench‐Step
SBP	127.9 ± 19.9	118.8 ± 9.6	123.4 ± 9.7	124.0 ± 13.3
(mmHg)
DBP	64.9 ± 6.4	63.2 ± 5.3	65.7 ± 6.2	64.5 ± 5.8
(mmHg)
VO2	33.2 ± 5.0	20.5 ± 4.8 *	23.2 ± 3.9	32.6 ± 4.4
(mL∙kg-1∙min-1)		ES = 2.63
HR	179.2 ± 7.5	173.9 ± 11.7 *	164.5 ± 9.1	181.0 ± 7.0
(beats∙min-1)		ES = 0.74
BT	37.6 ± 0.5 *	37.6 ± 0.6 *	36.5 ± 0.4	37.2 ± 0.7
(ºC)	ES = 2.43	ES = 0.62
RPP	22856 ± 3423	20693 ± 2323	20308 ± 1799	22448 ± 2620
(mmHg∙bpm)
PSI	6.39 ± 1.25	6.19 ± 1.32 *	3.80 ± 1.18	5.66 ± 1.84
(0-10)		ES = 2.00
RPE	13.2 ± 1.4 *	15.7 ± 7.9 *	13.9 ± 1.1	14.5 ± 1.9
(6-20)	ES = 0.56	ES = 0.21

Legend: STEP_RES = bench-step and resistance exercise sequence; RES_STEP = resistance exercise and bench-step sequence; ES = Effect size; * = significantly different from the identical type of exercise in RES_STEP. The mean values and standard deviation are given for systolic blood pressure (SBP), diastolic blood pressure (DBP), oxygen uptake (VO.

The RES_STEP sequence promoted higher VO2 (p = 0 .020) and a lower HR (p = 0.015) during the resistance exercise; however, during bench-step aerobics exercise, HR values were similar for both STEP_RES and RES_STEP sequences. The body temperature was significantly higher at the STEP_RES session sequence during both bench-step aerobics (p = 0.029) and resistance (p < 0.001) exercise. The PSI was significantly higher during the later resistance exercise in the STEP_RES sequence (p < 0.001). The RPE was significantly higher during the later performed type of exercise despite the sequence of the session; during bench-step aerobics (p = 0.003) in the RES_STEP sequence, and during resistance exercise (p = 0.018) in the STEP_RES sequence (Table 3).

The session sequence did not affect the post-exercise response of SBP (F1, 12 = 1.3, p = 0.28), DBP (F1, 12 = 1.4, p = 0.261), HR (F1, 12 = 3.2, p = 0.101) and BT (F1, 12 = 1.8, p = 0.21). During the post-exercise period there were differences for SBP (F1, 12 = 26.1, p = 0.000, η2 = 0.685), DBP (F1, 12 = 5.6, p = 0.001, η2 = 0.317), HR (F1, 12 = 120.8, p = 0.000, η2 = 0.910) and BT (F1, 12 = 4.7, p = 0.003, η2 = 0.282) related to rest values. The post-hoc comparisons revealed that independently of the sequence, the SBP responses were lower than pre-exercise during all post-exercise periods (p ≤ 0.001), with a continuous decrease until P45, followed by a rise (Table 4). There were also differences between P15 and P30 (p = 0.047), P15 and P45 (p = 0.001) and P15 and P60 (p = 0.005). No significant differences were found in DBP responses between the pre-exercise period and P15, although a significant decrease in DBP to below pre-exercise value during the post-exercise periods P30, P45 and P60 was noted (p ≤ 0.05). In addition, there were differences between P15 and P30 (p = 0.012), P15 and P45 (p = 0.005) and P15 and P60 (p = 0.013).

Table 4

Blood pressure, cardiovascular, respiratory and perceptual responses during post-exercise for the inverse session sequence (STEP_RES and RES_STEP).

			STEP_RES					RES_STEP
Variables	Rest		Post‐Exercise			Rest		Post‐Exercise
		P15	P30	P45	P60		P15	P30	P45	P60
SBP	107.3 ± 7.4	100.0 ± 4.7	97.1 ± 7.5 a	96.6 ± 5.4 a	97.5 ± 5.8a	109.0 ± 6.4	102.5 ± 7.8	98.5 ± 7.5	96.5 ± 6.1	98 ± 6.2
(mmHg)			ES: a = 0.46	ES: a = 0.67	ES: a = 0.47
DBP	66.2 ± 9.1	66.1 ± 7.5	64.2 ± 7.0a	59.8 ± 4.0a	60.9 ± 4.7a	69.0 ± 7.0	68.9 ± 10.0	62.5 ± 5.7	64.5 ± 6.9	64.9 ± 8.6
(mmHg)			ES: a = 0.30	ES: a = 1.10	ES: a = 0.88
VO2 (mL∙kg-1∙min-1)	4.8 ± 1.0	6.8 ± 1.4	-	-	-	4.6 ± 1.1	6.6 ± 0.9	-	-	-
HR	81.0 ± 9.4	118.0 ± 12.2	97.2 ± 11.1a	92.8 ± 10.8a b	90.2 ± 11.1a b	77.3 ± 8.4	115.0 ± 12.5	92.3 ± 12.1	89.6 ± 10.0	86.3 ± 12.8
(beats∙min-1)			ES: a = 1.78	ES: a = 2.19,	ES: a = 2.40,
				ES: b = 0.40	ES: b = 0.63
BT	36.9 ± 0.3	36.9 ± 0.04	36.8 ± 0.5	36.5 ± 0.5a b	36.5 ± 0.5a b	36.9 ± 0.4	36.7 ± 0.2	36.6 ± 0.4	36.4 ± 0.5	36.3 ± 0.3
(ºC)				ES: a = 1.13,	ES: a = 1.13,
				ES: b = 0.85	ES: b = 0.85

Legend: STEP_RES = bench-step and resistance exercise sequence; RES_STEP = resistance exercise and bench-step sequence; P15 = first 15min post-exercise period; P30 = second 15min post-exercise period; P45 = third 15min post-exercise period; P60 = fourth 15min post-exercise period; ES = Effect size; < 0.05; < 0.05. The mean values and standard deviation are given for systolic blood pressure (SBP), diastolic blood pressure (DBP), oxygen uptake (VO.

Independently of the session sequence, the HR was also significantly different between P15 and the following post-exercise periods (P30, P45 and P60, p ≤ 0.05), and between P30 and P45 (p = 0.001). The pre-exercise body temperature remained similar to P15 and P30, but different during P45 (p = 0.026) and P60 (p = 0.003). Also, there were differences between P15 and P45 (p = 0.018), P15 and P60 (p = 0.002), P30 and P45 (p = 0.044) and P30 and P60 (p = 0.017).

Discussion

This study revealed that the two sequences (STEP_RES and RES_STEP) promoted different responses in VO2, HR, body temperature, PSI and RPE, yet similar responses in exercise BP and also during the 60 min of recovery. The VO2 and HR responses were similar during bench-step exercise, which was performed at similar HR values (179 ± 8 bpm in STEP_RES sequence and 181 ± 7 bpm in RES_STEP sequence). However, during the resistance exercise of the RES_STEP sequence, the VO2 responses were 23.3 ± 3.9 mL∙kg-1∙min-1, significantly higher than the 20.5 ± 4.8 mL∙kg-1∙min-1 of STEP_RES sequence. This greater VO2 probably resulted from the higher total number of repetitions performed (reps = 169 for RES_STEP and reps = 147 for STEP_RES), the inexistence of previous muscle fatigue and the pattern of oxygen kinetics. The higher HR responses in the resistance exercise in the STEP_RES sequence may be due to the sympathetic nervous system maintenance of the previous bench-step aerobic exercise. During the resistance exercise, a higher HR was associated with lower VO2 in the STEP_RES sequence. It appears that the previous vigorous bench-step aerobics type exercise affected the low-to-moderate resistance exercise and that the chosen intensity for the preceding type of exercise is an important issue to consider. In another study with low (60% 8-RM) and high intensity resistance exercise (90% 8-RM) followed by cardiovascular exercise, VO2 during the subsequent type of exercise was higher only after the high-intensity resistance exercise (Kang et al., 2009). Therefore, it seems that for energy expenditure benefits, resistance exercise performed before the cardiovascular exercise needs to be intense.

Excess post-exercise VO2 and the post-exercise HR were higher during one-hour in both sequences, but the magnitude of change was similar in both exercise sequences. The slightly higher pattern in post-exercise VO2 and HR in the STEP_RES sequence did not achieve statistical significance. Usually, short-term excess post-exercise VO2 is higher after high-intensity resistance exercise and is related to higher metabolic disruption (Borsheim and Bahr, 2003). In this study, the resistance exercise intensity did not promote a higher post-exercise response.

The exercise BP increases were of small magnitude. During bench-step aerobics the SBP was 127.9 mmHg (STEP_RES) and 124.0 mmHg (RES_STEP) and after resistance exercise, it was 118.8 mmHg (STEP_RES) and 124.0 mmHg (RES_STEP). Despite the timing of the measurement of the exercise BP (i.e. immediately after the end of each exercise), that can aggregate exercise and post-exercise BP responses, the resistance exercise was performed with a low load and high-volume dynamic contractions, promoting smaller exercise BP increases compared to traditional resistance exercise responses (Figueroa and Vicil, 2011; MacDonald, 2002). It should also be emphasized that in this study there were no specific weight-training procedures that promote exaggerated increases in muscle metaboreflex and mechanoreflex (mechanical compression of the blood vessels in the exercising muscle beds), and the Valsalva manoeuvre, which significantly increases intrathoracic pressure (Linsenbardt et al., 1992).

In the post-exercise period, the sequence effect (STEP_RES or RES_STEP) did not affect the acute BP responses, however, there were post-exercise positive effects, possibly related to the total load. In fact, despite the sequence, SBP and DBP responses during recovery were significantly lower than pre-exercise and achieved a lower value at P45 (except for DBP in RES_STEP, with a lower value at P30). Also, the results revealed that the STEP_RES and RES_STEP sequences induced acute similar decreases in SBP (10.7 ± 2.0 and 12.5 ± 0.3 mmHg, respectively) and DBP (6.4 ± 5.1 and 6.5 ± 1.3 mmHg, respectively) compared to rest. These results indicate that the higher magnitude of post-exercise BP in normotensive participants is attained before one-hour after exercise and longer duration of post-exercise measures is needed to determine the duration of the post-exercise hypotensive effect. The cardiovascular exercise intensity used in this study was nearly 80% of HRR, therefore, requiring the use of a significant muscle mass that can lead to great hemodynamic stress, higher levels of circulating catecholamine's, greater cardiac output, increased blood flow and BP, and, therefore, greater stress (MacDonald, 2002). These factors lead to sustained vasodilation in active skeletal muscle, associated with a reduction in peripheral vascular resistance (enhanced by endothelial vasodilators such as nitric oxide, adenosine, prostaglandins, and thermodilatation), that affect the vasoconstrictor response to α-adrenergic stimulation (Forjazet al., 1998; MacDonald, 2002; Smelker et al., 2004), promoting a BP decrease after exercise (MacDonald, 2002).

This combined aerobic and resistance exercise effect on post-exercise BP was already identified (Keese et al., 2011, 2012; Ruiz et al., 2011), as well as the lower post-exercise BP responses related to increased intensity of later aerobic exercise (50%, 65% and 80% of VO2 peak) (Keese et al., 2012). However, the sequence effect (i.e. aerobic followed by resistance or resistance followed by aerobic) is quite unknown in the current literature. The post-exercise BP reduction in RES_STEP was probably related to the total load and, thus, not affected by the sequence.

The body temperature was higher during both bench-step and resistance exercise in the STEP_RES sequence than in the RES_STEP sequence. The effect of high-intensity and high muscle mass mobilization in the bench-step exercise was probably maintained during the following resistance exercise, therefore, it started with higher body temperature. This cumulative effect was already identified in other studies and variables when a more intense exercise was performed first (Kang et al., 2003, 2009). Greater post-exercise oxygen uptake (Kang et al., 2003, 2009), HR, and fat oxidation (Kang et al., 2003) were found in a high-to-low exercise intensity sequence. The PSI, that reflects the combined load of the thermoregulatory and cardiovascular systems, was higher in resistance exercise only when performed after aerobic bench-step. It seems that the effect of previous bench-step aerobics type exercise on both thermoregulatory and cardiovascular responses, was maintained in the following responses to exercise.

The RPE was higher during the last type of exercise, independently of the session sequence, revealing an important cumulative effect. This fact is supported by the higher RPE values reported in the resistance exercise when performed after the bench-step aerobic exercise, compared with the same load performed before cardiovascular exercise (15.7 ± 7.9 for STEP_RES vs. 13.9 ± 1.1 for RES_STEP). This effect is possibly explained by the previous exercise effects, like glycogen depletion, elevated blood lactate, HR and metabolites, as well as high secretion of growth hormone, catecholamines and cortisol (Kang et al., 2003). For optimal benefits specifically related to the lower exercise RPE, the type of exercise performed with higher intensity (or higher perceived exertion), could be performed earlier in the exercise session. This idea has already been presented, yet should be taken with caution (Kang et al., 2003).

In conclusion, the inverse sequence of bench-step aerobics and resistance exercise did not affect the BP responses neither during nor after exercise, but did affect VO2, HR, body temperature, PSI and RPE responses. When starting with bench-step aerobics, there was a higher body temperature maintained during the subsequent resistance exercise, probably because of the vigorous intensity and the large amount of muscle mass involved. Accordingly, resistance exercise promoted lower VO2, a higher HR and physiologic strain index. In both cases, the exercise performed first was pointed out with a lower perceived exertion. Despite the different sequences, this exercise load combination promoted an acute post-exercise BP decrease, for at least one-hour in these normotensive participants.