Ya-Ru Wang1,2, Jia-Song Zhang1, Ran Wang3, You-Ming Hou2, Hu-Aying Fu1, Yuan Xie1, San-Ji Gao1, Jin-da Wang1. 1. National Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou, 350002, P. R. China. 2. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops，Key Lab of Biopesticide and Chemical Biology, Ministry of Education & Fujian Province Key Laboratory of Insect Ecology, College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou, 350002, P.R. China. 3. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100012, P. R. China.
Abstract
BACKGROUND: Sugarcane is the most important sugar crops in the world. Like other crops, sugarcane is also suffered from herbivorous insect attack. The oriental armyworm Mythimna separata is a devastating pest of various crop in northeast Asia, outbreak of this pest resulted in substantially yield loss including sugarcane. However, the plant defense response situation is widely acquisition in model crops, but little information about how sugarcane plants defend themselves against this herbivore at the molecular and biochemical levels is studied. RESULTS: We combined transcriptome and metabolomics analysis to investigate the changes in gene expression and metabolic processes that occurred in sugarcane plants after continuous feeding by M. separata larvae for 12, and 24 h. We identified 13 662 genes and 55 metabolites that were differentially regulated in sugarcane plants fed upon by M. separata. The genes involved in phytohormones, transcription factors, and kinase-related were activated and the metabolism compounds such as carbohydrate, amino acid, ferulic substances and glutathione were detected regulated in sugarcane defense response. Comparable analyses showed a close correspondence relationship among pathways of phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis in transcript and metabolite profiles. Furthermore, a bioassay experiment was conducted to test the influence of up-regulated metabolites on M. separata growth and found chlorogenic acid showed lethal effect. CONCLUSION: The results of our study greatly enhanced understanding on sugarcane induced defense response mechanism against herbivore infestation at the transcriptional and metabolic levels. Also make contributions to provide clues for development of green pest control method. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
BACKGROUND:Sugarcane is the most important sugar crops in the world. Like other crops, sugarcane is also suffered from herbivorous insect attack. The oriental armywormMythimna separata is a devastating pest of various crop in northeast Asia, outbreak of this pest resulted in substantially yield loss including sugarcane. However, the plant defense response situation is widely acquisition in model crops, but little information about how sugarcane plants defend themselves against this herbivore at the molecular and biochemical levels is studied. RESULTS: We combined transcriptome and metabolomics analysis to investigate the changes in gene expression and metabolic processes that occurred in sugarcane plants after continuous feeding by M. separata larvae for 12, and 24 h. We identified 13 662 genes and 55 metabolites that were differentially regulated in sugarcane plants fed upon by M. separata. The genes involved in phytohormones, transcription factors, and kinase-related were activated and the metabolism compounds such as carbohydrate, amino acid, ferulic substances and glutathione were detected regulated in sugarcane defense response. Comparable analyses showed a close correspondence relationship among pathways of phenylalanine metabolism, phenylpropanoid biosynthesis, and flavonoid biosynthesis in transcript and metabolite profiles. Furthermore, a bioassay experiment was conducted to test the influence of up-regulated metabolites on M. separata growth and found chlorogenic acid showed lethal effect. CONCLUSION: The results of our study greatly enhanced understanding on sugarcane induced defense response mechanism against herbivore infestation at the transcriptional and metabolic levels. Also make contributions to provide clues for development of green pest control method. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
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Keywords:
M. separata; RNA sequencing; metabolism; phenylpropanoids; sugarcane