Fan Yang1,2,3, Liang Liu1,2,3, Shan Qiang4, Ching Yuan Hu1,2,3,5, Ying Li6, Yong Hong Meng7,8,9. 1. Engineering Research Center of High-Valued Utilization of Fruit Resources in Western China, Ministry of Education, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. 2. National Research & Development Center of Apple Processing Technology, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. 3. College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. 4. Xian Healthful Biotechnology Co., Ltd.,, Hangtuo Road, Changan, Xian, 710100, People's Republic of China. 5. Department of Human Nutrition, Food and Animal Sciences, College of Tropical Agriculture and Human Resources, University of Hawaii at Manoa, 1955 East-West Road, AgSci. 415J, Honolulu, HI, 96822, USA. 6. Department of Microbiology and Immunology, China Agricultural University, Haidian District, Beijing, 100193, People's Republic of China. 7. Engineering Research Center of High-Valued Utilization of Fruit Resources in Western China, Ministry of Education, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. mengyonghong@snnu.edu.cn. 8. National Research & Development Center of Apple Processing Technology, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. mengyonghong@snnu.edu.cn. 9. College of Food Engineering and Nutritional Science, Shaanxi Normal University, 620 West Changan Avenue, Changan, Xian, 710119, People's Republic of China. mengyonghong@snnu.edu.cn.
Abstract
OBJECTIVE: β-Carotene has been widely used in the food and feed industry and has significant commercial value. This study aimed to increase the β-carotene production in engineered Yarrowia lipolytica by optimizing the host metabolic network. The DID2 gene, a subunit of the endosomal sorting complex required for transport (ESCRT), was integrated into a β-carotene producing strain. RESULTS: The β-carotene production was increased by 260%, and the biomass increased by 10% for engineered Y. lipolytica. Meanwhile, DID2 elevated the mRNA level and protein level of the genes in the β-carotene synthesis pathway, then increased precursors (FPP, Lycopene) utilization. DID2 also increased the mRNA level of the genes in the glucose pathway, pentose phosphate pathway, and tricarboxylic acid cycle and promoted glucose utilization and cofactors consumption. CONCLUSION: The ESCRT protein complex subunit, DID2, improved β-carotene production in engineered Y. lipolytica and beneficial to glucose utilization and cofactors consumption. This study provided new finding of the DID2 gene's function and it mostly could be used for many other natural product productions.
OBJECTIVE: β-Carotene has been widely used in the food and feed industry and has significant commercial value. This study aimed to increase the β-carotene production in engineered Yarrowia lipolytica by optimizing the host metabolic network. The DID2 gene, a subunit of the endosomal sorting complex required for transport (ESCRT), was integrated into a β-carotene producing strain. RESULTS: The β-carotene production was increased by 260%, and the biomass increased by 10% for engineered Y. lipolytica. Meanwhile, DID2 elevated the mRNA level and protein level of the genes in the β-carotene synthesis pathway, then increased precursors (FPP, Lycopene) utilization. DID2 also increased the mRNA level of the genes in the glucose pathway, pentose phosphate pathway, and tricarboxylic acid cycle and promoted glucose utilization and cofactors consumption. CONCLUSION: The ESCRT protein complex subunit, DID2, improved β-carotene production in engineered Y. lipolytica and beneficial to glucose utilization and cofactors consumption. This study provided new finding of the DID2 gene's function and it mostly could be used for many other natural product productions.