Nawal E Al-Hazmi1, Deyala M Naguib2,3. 1. Department of Chemistry, Division of Biology (Microbiology), University College of Qunfudhah, Umm Al-Qura University, Qunfudhah, Saudi Arabia. 2. Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt. dmna2610science@yahoo.com. 3. Biology Department, Faculty of Science and Arts in Qilwah, Albaha University, Qilwah, Saudi Arabia. dmna2610science@yahoo.com.
Abstract
OBJECTIVES: To discover new natural effective anticancer agents and new antibacterial agents against antibiotic-resistant bacteria which are the most serious public health concern. Another important concern is drug delivery which is the transport of pharmaceutical compounds to have a therapeutic effect in organisms having a disease. Azurin is a promising anticancer agent produced from Pseudomonas aeruginosa. This study tried to test the effectiveness of the immobilization of azurin on nano-chitosan to enhance its anticancer and antibacterial activity against gastrointestinal cancer and its related bacteria. METHODS: We purified azurin protein from Pseudomonas aeruginosa and then immobilized it on nano-chitosan. The anticancer activity of the free and nano-azurin is tested against a gastric cancer cell line (CLS-145), pancreatic cancer cell line (AsPC-1), colon cancer cell line (HCT116), esophagus cancer cell line (KYSE-410), and liver cancer cell line (HepG2). The antibacterial activity of both free and immobilized azurin also is tested against bacterial species related to the gastrointestinal cancer biopsies: Helicobacter pylori, Bacteroides fragilis, Salmonella enterica, Fusobacterium nucleatum, and Porphyromonas gingivalis. RESULTS: Both free and nano-azurin showed high anticancer and antibacterial activity. Immobilization significantly increased the anticancer and antibacterial activity of the azurin CONCLUSION: Nano-azurin can be used as an effective anticancer and antibacterial agent against gastrointestinal cancer and bacterial species related to these cancers.
OBJECTIVES: To discover new natural effective anticancer agents and new antibacterial agents against antibiotic-resistant bacteria which are the most serious public health concern. Another important concern is drug delivery which is the transport of pharmaceutical compounds to have a therapeutic effect in organisms having a disease. Azurin is a promising anticancer agent produced from Pseudomonas aeruginosa. This study tried to test the effectiveness of the immobilization of azurin on nano-chitosan to enhance its anticancer and antibacterial activity against gastrointestinal cancer and its related bacteria. METHODS: We purified azurin protein from Pseudomonas aeruginosa and then immobilized it on nano-chitosan. The anticancer activity of the free and nano-azurin is tested against a gastric cancer cell line (CLS-145), pancreatic cancer cell line (AsPC-1), colon cancer cell line (HCT116), esophagus cancer cell line (KYSE-410), and liver cancer cell line (HepG2). The antibacterial activity of both free and immobilized azurin also is tested against bacterial species related to the gastrointestinal cancer biopsies: Helicobacter pylori, Bacteroides fragilis, Salmonella enterica, Fusobacterium nucleatum, and Porphyromonas gingivalis. RESULTS: Both free and nano-azurin showed high anticancer and antibacterial activity. Immobilization significantly increased the anticancer and antibacterial activity of the azurin CONCLUSION: Nano-azurin can be used as an effective anticancer and antibacterial agent against gastrointestinal cancer and bacterial species related to these cancers.
Keywords:
Bacteroides fragilis; Colon cancer cell line (HCT116); Esophagus cancer cell line (KYSE-410); Fusobacterium nucleatum; Gastric cancer cell line (CLS-145); Liver cancer cell line (HepG2). Helicobacter pylori; Pancreatic cancer cell line (AsPC-1); Porphyromonas gingivalis; Salmonella enterica
Authors: Fatma Vatansever; Wanessa C M A de Melo; Pinar Avci; Daniela Vecchio; Magesh Sadasivam; Asheesh Gupta; Rakkiyappan Chandran; Mahdi Karimi; Nivaldo A Parizotto; Rui Yin; George P Tegos; Michael R Hamblin Journal: FEMS Microbiol Rev Date: 2013-07-25 Impact factor: 16.408
Authors: Rajeshwari R Mehta; Tohru Yamada; Brad N Taylor; Konstantin Christov; Marissa L King; Dibyen Majumdar; Fatima Lekmine; Chinnaswamy Tiruppathi; Anne Shilkaitis; Laura Bratescu; Albert Green; Craig W Beattie; Tapas K Das Gupta Journal: Angiogenesis Date: 2011-06-11 Impact factor: 9.596
Authors: Z Flachbartova; L Pulzova; E Bencurova; L Potocnakova; L Comor; Z Bednarikova; M Bhide Journal: Microbiol Res Date: 2016-04-27 Impact factor: 5.415