Yannan Zhang1, Huanzhen Zhang2, Shengzhong Rong3, Cailing Bian4, Yuexin Yang5, Hongzhi Pan6. 1. Department of Nutrition and Food Hygiene, School of Public Health and Management, Ningxia Medical University, Yinchuan, 750004, China; Ningxia Key Laboratory of Environmental Factors and Chronic Disease Control, Ningxia Medical University, Yinchuan, 750004, China. 2. Department of Obstetrics and Gynecology, Tai'an Hospital of Traditional Chinese Medicine, Tai'an, 271000, China. 3. Public Health School, Mudanjiang Medical University, Mudanjiang, Heilongjiang, 157011, China. 4. Tai'an City Central Hospital, Tai'an, 271000, China. 5. National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing, 100050, China. Electronic address: xyang@263.net. 6. Collaborative Research Center, Shanghai University of Medicine and Health Sciences, Pudong, Shanghai, 201318, China. Electronic address: panhongzhi@163.com.
Abstract
BACKGROUND AND AIMS: Hyperuricemia (HUA) were associated with Metabolic syndrome (MetS) and its components. However, the molecular mechanism of uric acid in the development of MetS was not well elucidated. The aim of this study was developing a systemic metabolic profile by using metabolomics approach to explore the molecular mechanism of uric acid in the development of MetS. METHODS AND RESULTS: Anthropometric, clinical biochemical data, and serum samples were collected from patients with MetS, MetS combined with HUA (MetS & HUA) and healthy controls. 1H nuclear magnetic resonance (NMR) spectroscopy was used to detect endogenous small molecule metabolites of serum samples, then multivariate statistical analysis was applied to distinguish samples of different groups. In addition, pathway analysis was performed to contribute to understanding the metabolic change. By serum metabolic profiling, a total of 20 identified metabolites including lipids, amino acids, and organic acids were significantly altered in MetS and MetS & HUA patients. MetS & HUA patients presented a more severe disorder in both identified metabolites and BMI and biochemical indexes. According to pathway analysis, there were 3 and 5 metabolic pathways remarkably perturbed in MetS and MetS & HUA group respectively. CONCLUSION: Taken together, we identified disordered metabolites and related pathways for both MetS and MetS & HUA patients, and found a more severe metabolic disorder in MetS patients who has a higher serum uric acid. Our study provides biochemical insights into the metabolic alteration for the progress of MetS.
BACKGROUND AND AIMS: Hyperuricemia (HUA) were associated with Metabolic syndrome (MetS) and its components. However, the molecular mechanism of uric acid in the development of MetS was not well elucidated. The aim of this study was developing a systemic metabolic profile by using metabolomics approach to explore the molecular mechanism of uric acid in the development of MetS. METHODS AND RESULTS: Anthropometric, clinical biochemical data, and serum samples were collected from patients with MetS, MetS combined with HUA (MetS & HUA) and healthy controls. 1H nuclear magnetic resonance (NMR) spectroscopy was used to detect endogenous small molecule metabolites of serum samples, then multivariate statistical analysis was applied to distinguish samples of different groups. In addition, pathway analysis was performed to contribute to understanding the metabolic change. By serum metabolic profiling, a total of 20 identified metabolites including lipids, amino acids, and organic acids were significantly altered in MetS and MetS & HUA patients. MetS & HUA patients presented a more severe disorder in both identified metabolites and BMI and biochemical indexes. According to pathway analysis, there were 3 and 5 metabolic pathways remarkably perturbed in MetS and MetS & HUA group respectively. CONCLUSION: Taken together, we identified disordered metabolites and related pathways for both MetS and MetS & HUA patients, and found a more severe metabolic disorder in MetS patients who has a higher serum uric acid. Our study provides biochemical insights into the metabolic alteration for the progress of MetS.
Authors: Leen Oyoun Alsoud; Nelson C Soares; Hamza M Al-Hroub; Muath Mousa; Violet Kasabri; Nailya Bulatova; Maysa Suyagh; Karem H Alzoubi; Waseem El-Huneidi; Bashaer Abu-Irmaileh; Yasser Bustanji; Mohammad H Semreen Journal: Metabolites Date: 2022-06-01