María Angela Jure1, Leonardo Albarracin2, Juan Martin Vargas1, Stefania Dentice Maidana3, Juan Cortez Zamar4, Haruki Kitazawa5, Julio Villena6. 1. Laboratory of Antimicrobials, Institute of Microbiology 'Luis C. Verna', Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Tucumán, Argentina. 2. Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Scientific Computing Laboratory, Computer Science Department, Faculty of Exact Science and Technology, National University of Tucumán, Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 3. Laboratory of Antimicrobials, Institute of Microbiology 'Luis C. Verna', Faculty of Biochemistry, Chemistry and Pharmacy, National University of Tucumán, Tucumán, Argentina; Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. 4. Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina. 5. Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan; Livestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. Electronic address: haruki.kitazawa.c7@tohoku.ac.jp. 6. Laboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucumán, Argentina; Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan. Electronic address: jcvillena@cerela.org.ar.
Abstract
OBJECTIVES: The emergence and spread of hypermucoviscous KPC-2-producing Klebsiella pneumoniae strains belonging to the sequence type 25 (ST25) clone was reported recently in Northwest Argentina as a leading cause of nosocomial infections. The aim of this work was to perform whole-genome sequencing (WGS) to analyse antimicrobial resistance genes (ARGs), virulence factors and colonisation-associated genes in two carbapenem-resistant KPC-2-producing ST25 K. pneumoniae strains isolated from hospitalised patients. METHODS: Classical microbiological methods were applied to recover K. pneumoniae LABACER 01 from a bone sample and LABACER 27 from the respiratory tract of two hospitalised patients. Bacteria were identified by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF). WGS was performed using an Illumina MiSeq platform. Genome annotation and analysis were performed with available databases and bioinformatic tools. RESULTS: Genomic analysis revealed a genome of 5 598 020 bp with 19 further characterised ARGs in strain LABACER 01, and a genome of 5 622 382 bp with 20 ARGs in strain LABACER 27. Bioinformatics analysis also predicted genomic regions associated with virulence factors and mucosal tissue colonisation. CONCLUSION: This study reports the genomic analysis of K. pneumoniae LABACER 01 and LABACER 27, two hypermucoviscous carbapenem-resistant ST25 strains, which expands our knowledge on the antibiotic resistance, pathogenic mechanisms and biology of ST25 clones recently emerging in Argentina.
OBJECTIVES: The emergence and spread of hypermucoviscous KPC-2-producing Klebsiella pneumoniae strains belonging to the sequence type 25 (ST25) clone was reported recently in Northwest Argentina as a leading cause of nosocomial infections. The aim of this work was to perform whole-genome sequencing (WGS) to analyse antimicrobial resistance genes (ARGs), virulence factors and colonisation-associated genes in two carbapenem-resistant KPC-2-producing ST25 K. pneumoniae strains isolated from hospitalised patients. METHODS: Classical microbiological methods were applied to recover K. pneumoniae LABACER 01 from a bone sample and LABACER 27 from the respiratory tract of two hospitalised patients. Bacteria were identified by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF). WGS was performed using an Illumina MiSeq platform. Genome annotation and analysis were performed with available databases and bioinformatic tools. RESULTS: Genomic analysis revealed a genome of 5 598 020 bp with 19 further characterised ARGs in strain LABACER 01, and a genome of 5 622 382 bp with 20 ARGs in strain LABACER 27. Bioinformatics analysis also predicted genomic regions associated with virulence factors and mucosal tissue colonisation. CONCLUSION: This study reports the genomic analysis of K. pneumoniae LABACER 01 and LABACER 27, two hypermucoviscous carbapenem-resistant ST25 strains, which expands our knowledge on the antibiotic resistance, pathogenic mechanisms and biology of ST25 clones recently emerging in Argentina.
Authors: Leonardo Albarracin; Ramiro Ortiz Moyano; Juan Martin Vargas; Bruno G N Andrade; Juan Cortez Zamar; Stefania Dentice Maidana; Kohtaro Fukuyama; Shoichiro Kurata; María Ángela Jure; Haruki Kitazawa; Julio Villena Journal: Int J Mol Sci Date: 2022-07-01 Impact factor: 6.208