Brian M Schmidt1,2, John Erb-Downward3, Piyush Ranjan3, Robert Dickson3,4,5. 1. Division of Metabolism, Endocrinology & Diabetes, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, 48109, USA. bmcs@med.umich.edu. 2. University of Michigan Medical School, 24 Frank Lloyd Wright Drive Lobby C, Ann Arbor, MI, 48109, USA. bmcs@med.umich.edu. 3. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA. 4. Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA. 5. Michigan Center for Integrative Research in Critical Care, Ann Arbor, MI, USA.
Abstract
PURPOSE OF REVIEW: Diabetes mellitus may affect every third adult American by 2050, and about one-third will develop a diabetic foot ulcer (DFU) during their lifetime. The current standard of care results in healing of less than 50% of all DFUs. Many individuals with DFU develop limb-threatening infection which place them at risk for additional morbidity and mortality. We review research associated with culture-independent next-generation sequencing techniques pertaining to diabetic foot ulcers and their potential for clinical application. RECENT FINDINGS: Diabetic foot ulcers are a growing problem and clinicians are limited by their reliance on conventional culture. Metagenomic sequencing technology provides an unparalleled viewpoint of the polymicrobial constituency of DFU. The microbiome techniques used to study the microbial constituency of DFU may offer insight to improve care for these patients, but without standardized approaches in research based on real-world clinical practices, a significant knowledge gap will remain.
PURPOSE OF REVIEW: Diabetes mellitus may affect every third adult American by 2050, and about one-third will develop a diabetic foot ulcer (DFU) during their lifetime. The current standard of care results in healing of less than 50% of all DFUs. Many individuals with DFU develop limb-threatening infection which place them at risk for additional morbidity and mortality. We review research associated with culture-independent next-generation sequencing techniques pertaining to diabetic foot ulcers and their potential for clinical application. RECENT FINDINGS:Diabetic foot ulcers are a growing problem and clinicians are limited by their reliance on conventional culture. Metagenomic sequencing technology provides an unparalleled viewpoint of the polymicrobial constituency of DFU. The microbiome techniques used to study the microbial constituency of DFU may offer insight to improve care for these patients, but without standardized approaches in research based on real-world clinical practices, a significant knowledge gap will remain.
Authors: Lee C Rogers; Robert G Frykberg; David G Armstrong; Andrew J M Boulton; Michael Edmonds; Georges Ha Van; Agnes Hartemann; Frances Game; William Jeffcoate; Alexandra Jirkovska; Edward Jude; Stephan Morbach; William B Morrison; Michael Pinzur; Dario Pitocco; Lee Sanders; Dane K Wukich; Luigi Uccioli Journal: J Am Podiatr Med Assoc Date: 2011 Sep-Oct
Authors: Benjamin A Lipsky; Javier Aragón-Sánchez; Mathew Diggle; John Embil; Shigeo Kono; Lawrence Lavery; Éric Senneville; Vilma Urbančič-Rovan; Suzanne Van Asten; Edgar J G Peters Journal: Diabetes Metab Res Rev Date: 2016-01 Impact factor: 4.876
Authors: Kristy Pickwell; Volkert Siersma; Marleen Kars; Jan Apelqvist; Karel Bakker; Michael Edmonds; Per Holstein; Alexandra Jirkovská; Edward Jude; Didac Mauricio; Alberto Piaggesi; Gunnel Ragnarson Tennvall; Heinrich Reike; Maximilian Spraul; Luigi Uccioli; Vilma Urbancic; Kristien van Acker; Jeff van Baal; Nicolaas Schaper Journal: Diabetes Care Date: 2015-02-09 Impact factor: 19.112
Authors: L Prompers; M Huijberts; J Apelqvist; E Jude; A Piaggesi; K Bakker; M Edmonds; P Holstein; A Jirkovska; D Mauricio; G Ragnarson Tennvall; H Reike; M Spraul; L Uccioli; V Urbancic; K Van Acker; J van Baal; F van Merode; N Schaper Journal: Diabetologia Date: 2006-11-09 Impact factor: 10.122
Authors: Rodica Pop-Busui; Andrew J M Boulton; Eva L Feldman; Vera Bril; Roy Freeman; Rayaz A Malik; Jay M Sosenko; Dan Ziegler Journal: Diabetes Care Date: 2017-01 Impact factor: 19.112