Yihui Fu1, Lirong Liu1, Jiabin Zhan2, Huijuan Zhan3, Chun Qiu4. 1. Department of Respiratory and Critical Care Medicine, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University) Haikou 570311, China. 2. Department of Otorhinolaryngology Head and Neck Surgery, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University) Haikou 570311, China. 3. Department of Pharmacy, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University) Haikou 570311, China. 4. Department of Oncology, Hainan General Hospital (Hainan Affiliated Hospital of Hainan Medical University) Haikou 570311, China.
Abstract
OBJECTIVE: This research explored and analyzed LncRNA GAS5 expression in non-small cell lung cancer (NSCLC) tissues and its correlation with Ki67 and EGFR. METHODS: A total of 130 samples of paraffin-embedded NSCLC tissues and para-cancerous normal tissues that were collected in the Department of Pathology from January 2014 to April 2016 were selected. The relative expression of LncRNA GAS5 and Ki67/EGFR in both NSCLC tissues and para-cancerous normal tissues were detected via RT-PCR and immunohistochemistry respectively. Subsequently, we analyzed the relative expression of LncRNA GAS5, the expression of Ki67/EGFR and its correlation with clinicopathological features and prognosis of patients, and studied the correlation between LncRNA GAS5 and Ki67/EGFR. RESULTS: The relative expression of LncRNA GAS5 in NSCLC tissues was substantially less than that of the para-cancerous normal tissues (P<0.05). The positive expression rate of Ki67/EGFR in NSCLC tissues remarkably exceeded that in para-cancerous normal tissues (P<0.05). The relative expression of LncRNA GAS5 was correlated with the degree of tumor differentiation, TNM staging and lymph node metastasis (P<0.05). The positive expression rate of Ki67 and EGFR in NSCLC tissues was related to TNM stage and metastasis of lymph node (P<0.05). In addition, the survival of patients with high LncRNA GAS5 expression was obviously superior to those with low LncRNA GAS5 expression (P<0.05), patients with negative Ki67 had superior survival than those with positive Ki67 (P<0.05), and patients with negative EGFR had increased survival over those with positive EGFR (P<0.05). Moreover, the positive rates of Ki67 and EGFR in patients with low LncRNA GAS5 expressions were obviously higher than those with high LncRNA GAS5 expressions (P<0.05). The relative expression level of LncRNA GAS5 in NSCLC patients had a remarkably negative correlation with Ki67 and EGFR (P<0.05). CONCLUSION: The decrease in LncRNA GAS5 expression and the over-express of Ki67/EGFR occur in NSCLC tissues, the expressions of LncRNA GAS5, Ki67 and EGFR are connected with the progression, metastasis and prognosis of tumor; and LncRNA GAS5 is related to the expression of Ki67 and EGFR. These three factors are involved in the tumorigenesis and growth of the NSCLC process. AJTR
OBJECTIVE: This research explored and analyzed LncRNA GAS5 expression in non-small cell lung cancer (NSCLC) tissues and its correlation with Ki67 and EGFR. METHODS: A total of 130 samples of paraffin-embedded NSCLC tissues and para-cancerous normal tissues that were collected in the Department of Pathology from January 2014 to April 2016 were selected. The relative expression of LncRNA GAS5 and Ki67/EGFR in both NSCLC tissues and para-cancerous normal tissues were detected via RT-PCR and immunohistochemistry respectively. Subsequently, we analyzed the relative expression of LncRNA GAS5, the expression of Ki67/EGFR and its correlation with clinicopathological features and prognosis of patients, and studied the correlation between LncRNA GAS5 and Ki67/EGFR. RESULTS: The relative expression of LncRNA GAS5 in NSCLC tissues was substantially less than that of the para-cancerous normal tissues (P<0.05). The positive expression rate of Ki67/EGFR in NSCLC tissues remarkably exceeded that in para-cancerous normal tissues (P<0.05). The relative expression of LncRNA GAS5 was correlated with the degree of tumor differentiation, TNM staging and lymph node metastasis (P<0.05). The positive expression rate of Ki67 and EGFR in NSCLC tissues was related to TNM stage and metastasis of lymph node (P<0.05). In addition, the survival of patients with high LncRNA GAS5 expression was obviously superior to those with low LncRNA GAS5 expression (P<0.05), patients with negative Ki67 had superior survival than those with positive Ki67 (P<0.05), and patients with negative EGFR had increased survival over those with positive EGFR (P<0.05). Moreover, the positive rates of Ki67 and EGFR in patients with low LncRNA GAS5 expressions were obviously higher than those with high LncRNA GAS5 expressions (P<0.05). The relative expression level of LncRNA GAS5 in NSCLCpatients had a remarkably negative correlation with Ki67 and EGFR (P<0.05). CONCLUSION: The decrease in LncRNA GAS5 expression and the over-express of Ki67/EGFR occur in NSCLC tissues, the expressions of LncRNA GAS5, Ki67 and EGFR are connected with the progression, metastasis and prognosis of tumor; and LncRNA GAS5 is related to the expression of Ki67 and EGFR. These three factors are involved in the tumorigenesis and growth of the NSCLC process. AJTR