Di Li1, Jinwei Ren2, Tianxing Wang2, Lan Wu2, Peng Liu1, Yong Li2. 1. Department of Clinical Nutrition, Peking University People's Hospital Beijing 100044, PR China. 2. Department of Nutrition and Food Hygiene, School of Public Health, Peking University Beijing 100191, PR China.
Abstract
OBJECTIVE: To investigate the anti-hypoxia effects of walnut oligopeptides (WOPs) in mice. METHODS: Randomly divide the mice into 4 experimental sets. Then randomly divide each set of mice into 5 groups, including one vehicle control group, one whey protein group (220 mg/kg), and three WOPs intervention groups (110 mg/kg, 220 mg/kg, 440 mg/kg). Test substances were administered orally to mice via the drinking water for 30 days. RESULTS: WOPs significantly extended the normobaric hypoxia survival time, sodium nitrite toxicosis survival time, and acute cerebral ischemia survival time. Notably, WOPs increased red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) levels, decreased malonaldehyde (MDA) content and lactate content in brain, enhanced brain lactate dehydrogenase (LDH) activity, and promoted the expression levels of hypoxia-inducible factor 1alpha (HIF1α) mRNA and vascular endothelial growth factor (VEGF) mRNA. CONCLUSION: WOPs have anti-hypoxia effects, and the mechanism may involve the following aspects: the first is to improve the blood's oxygen carrying capacity and oxygen utilization rate, the second is to minimize the lesion of lipid peroxidation, the third is to increase the brain's ability to buffer against lactic acidosis of mice, and the fourth is to promote angiogenesis and regulate hypoxia response. AJTR
OBJECTIVE: To investigate the anti-hypoxia effects of walnut oligopeptides (WOPs) in mice. METHODS: Randomly divide the mice into 4 experimental sets. Then randomly divide each set of mice into 5 groups, including one vehicle control group, one whey protein group (220 mg/kg), and three WOPs intervention groups (110 mg/kg, 220 mg/kg, 440 mg/kg). Test substances were administered orally to mice via the drinking water for 30 days. RESULTS: WOPs significantly extended the normobaric hypoxia survival time, sodium nitritetoxicosis survival time, and acute cerebral ischemia survival time. Notably, WOPs increased red blood cell (RBC), hemoglobin (Hb) and hematocrit (Hct) levels, decreased malonaldehyde (MDA) content and lactate content in brain, enhanced brain lactate dehydrogenase (LDH) activity, and promoted the expression levels of hypoxia-inducible factor 1alpha (HIF1α) mRNA and vascular endothelial growth factor (VEGF) mRNA. CONCLUSION: WOPs have anti-hypoxia effects, and the mechanism may involve the following aspects: the first is to improve the blood's oxygen carrying capacity and oxygen utilization rate, the second is to minimize the lesion of lipid peroxidation, the third is to increase the brain's ability to buffer against lactic acidosis of mice, and the fourth is to promote angiogenesis and regulate hypoxia response. AJTR
Authors: Juan Wang; Lan Zhou Li; Yan Ge Liu; Li Rong Teng; Jia Hui Lu; Jing Xie; Wen Ji Hu; Yan Liu; Yang Liu; Di Wang; Le Sheng Teng Journal: Mol Med Rep Date: 2015-12-30 Impact factor: 2.952