Yi-Chen Juan1, Yee-Fun Su2, Chyi-Huey Bai2,3, Yen-Chun Fan3, Tzu-Tung Kuo3, Hui-Hsin Ko4,5,6, Hsin-Hui Peng4,5,6, Chun-Pin Chiang4,5,7,8, Chyng-Wen Fwu1, Shih-Jung Cheng4,5,9. 1. iStat Biomedical Co., Ltd, New Taipei City, Taiwan. 2. Department of Public Health, College of Medicine, Taipei Medical University, Taipei, Taiwan. 3. School of Public Health, College of Public Health, Taipei Medical University, Taipei, Taiwan. 4. Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan. 5. Department of Dentistry, National Taiwan University Hospital, College of Medicine, Taipei, Taiwan. 6. Department of Dentistry, National Taiwan University Hospital Hsin-Chu Branch, College of Medicine, Hsin-Chu, Taiwan. 7. Department of Dentistry, Far Eastern Memorial Hospital, New Taipei City, Taiwan. 8. Department of Dentistry, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan. 9. School of Dentistry, National Taiwan University, Taipei, Taiwan.
Abstract
OBJECTIVES: This hospital-based cohort study evaluated whether ZNF582 and PAX1 methylation levels at baseline can be used as biomarkers to identify lesions with a high potential for malignant transformation in patients with normal mucosa and oral potentially malignant disorders. PATIENTS AND METHODS: We recruited 171 adult patients with normal mucosa and oral potentially malignant disorders in 2012-2014. They were followed until 2017. Outcomes, including advanced histopathological findings and oral cancer occurrence, were obtained from medical charts, the Taiwan Cancer Registry, and Cause-of-death Data. Kaplan-Meier analysis and Cox proportional hazards regression models were used to examine the association of ZNF582 and PAX1 methylation levels at baseline with subsequent outcome occurrences. RESULTS: After 260 192 days of follow-up, 11 cases of oral cancer and 4 cases of advanced histopathological progression occurred. Patients with higher ZNF582 and PAX1 methylation levels at baseline had a higher incidence of disease progression. After adjustment for all studied factors using Cox proportional hazards regression models, ZNF582m level (adjusted hazard ratio, 11.41; 95% CI, 2.05-63.36; P = .005) was the only significant and independent predictor of disease progression. CONCLUSIONS: ZNF582 hypermethylation can be an effective and non-invasive biomarker for identifying oral lesions with a high potential for malignant transformation. This article is protected by copyright. All rights reserved.
OBJECTIVES: This hospital-based cohort study evaluated whether ZNF582 and PAX1 methylation levels at baseline can be used as biomarkers to identify lesions with a high potential for malignant transformation in patients with normal mucosa and oral potentially malignant disorders. PATIENTS AND METHODS: We recruited 171 adult patients with normal mucosa and oral potentially malignant disorders in 2012-2014. They were followed until 2017. Outcomes, including advanced histopathological findings and oral cancer occurrence, were obtained from medical charts, the Taiwan Cancer Registry, and Cause-of-death Data. Kaplan-Meier analysis and Cox proportional hazards regression models were used to examine the association of ZNF582 and PAX1 methylation levels at baseline with subsequent outcome occurrences. RESULTS: After 260 192 days of follow-up, 11 cases of oral cancer and 4 cases of advanced histopathological progression occurred. Patients with higher ZNF582 and PAX1 methylation levels at baseline had a higher incidence of disease progression. After adjustment for all studied factors using Cox proportional hazards regression models, ZNF582m level (adjusted hazard ratio, 11.41; 95% CI, 2.05-63.36; P = .005) was the only significant and independent predictor of disease progression. CONCLUSIONS:ZNF582 hypermethylation can be an effective and non-invasive biomarker for identifying oral lesions with a high potential for malignant transformation. This article is protected by copyright. All rights reserved.