| Literature DB >> 34140680 |
Lucia Lorenzi1,2, Hua-Sheng Chiu3, Francisco Avila Cobos1,2, Stephen Gross4, Pieter-Jan Volders1,2,5, Robrecht Cannoodt1,2,6,7,8, Justine Nuytens1,2, Katrien Vanderheyden1,2, Jasper Anckaert1,2, Steve Lefever1,2, Aidan P Tay9,10, Eric J de Bony1,2, Wim Trypsteen1,2, Fien Gysens1,2, Marieke Vromman1,2, Tine Goovaerts11, Thomas Birkballe Hansen12, Scott Kuersten4, Nele Nijs13, Tom Taghon14, Karim Vermaelen15, Ken R Bracke15, Yvan Saeys5,6, Tim De Meyer2,11, Nandan P Deshpande16, Govardhan Anande17,18, Ting-Wen Chen19, Marc R Wilkins16, Ashwin Unnikrishnan17,18, Katleen De Preter1,2, Jørgen Kjems12, Jan Koster20, Gary P Schroth4, Jo Vandesompele1,2, Pavel Sumazin21, Pieter Mestdagh22,23.
Abstract
Existing compendia of non-coding RNA (ncRNA) are incomplete, in part because they are derived almost exclusively from small and polyadenylated RNAs. Here we present a more comprehensive atlas of the human transcriptome, which includes small and polyA RNA as well as total RNA from 300 human tissues and cell lines. We report thousands of previously uncharacterized RNAs, increasing the number of documented ncRNAs by approximately 8%. To infer functional regulation by known and newly characterized ncRNAs, we exploited pre-mRNA abundance estimates from total RNA sequencing, revealing 316 microRNAs and 3,310 long non-coding RNAs with multiple lines of evidence for roles in regulating protein-coding genes and pathways. Our study both refines and expands the current catalog of human ncRNAs and their regulatory interactions. All data, analyses and results are available for download and interrogation in the R2 web portal, serving as a basis for future exploration of RNA biology and function.Entities:
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Year: 2021 PMID： 34140680 DOI： 10.1038/s41587-021-00936-1
Source DB: PubMed Journal: Nat Biotechnol ISSN： 1087-0156 Impact factor: 54.908