High-resolution melting curve FRET-PCR rapidly identifies SARS-CoV-2 mutations.

Reverse transcription FRET-PCRs were designed against two most common mutations in the SARS-CoV-2 (A23403G in the spike protein; C14408T in the RNA-dependent RNA polymerase). Based on high-resolution melting curve analysis, the RT FRET-PCRs identified the mutations in ATCC control viruses, and feline and human clinical samples. All major makes of PCR machines can perform melting curve analysis and thus further specifically designed FRET-PCRs could enable active surveillance for mutations and variants in countries where genome sequencing is not readily available. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.