Leny Sanchez1, Louisa A Messenger2, Tapan Bhattacharyya3, Robert H Gilman1,4, Holger Mayta1, Rony Colanzi5, Ricardo Bozo6, Manuela Verástegui1, Michael A Miles3, Caryn Bern7. 1. Laboratorio de Investigación en Enfermedades Infecciosas, Departamento de Ciencias Celulares y Moleculares, Universidad Peruana Cayetano Heredia, Lima Av. Honorio Delgado 430, San Martín de Porres 15102, Perú. 2. Department of Disease Control, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK. 3. Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK. 4. Department of International Health, Johns Hopkins Bloomberg School of Public Health, 615 N Wolfe St, Baltimore, Maryland 21205, USA. 5. Hospital Japonés de Tercer Nivel, Santa Cruz de la Sierra, Plurinational State of Bolivia. 6. Hospital Municipal Camiri, Camiri, Plurinational State of Bolivia. 7. Department of Epidemiology and Biostatistics, School of Medicine, University of California, 550 16th St, San Francisco, California 94158, USA.
Abstract
BACKGROUND: This study identified Trypanosoma cruzi discrete typing units (DTUs) in maternal and infant specimens collected from two hospitals in Bolivia, using conventional genotyping and DTU-specific serotyping. METHODS: Specimens from 142 mothers were used, including 24 seronegative and 118 seropositive individuals; 29 women transmitted T. cruzi to their infants. Maternal and infant parasite loads were determined by quantitative real-time PCR. Maternal sera were tested with an in-house parasite lysate ELISA and serotyped by a lineage-specific peptide ELISA, targeting the trypomastigote small surface antigen (TSSA). Trypanosoma cruzi genotypes in infected infants were determined by a triple PCR-RFLP assay. RESULTS: All infant specimens were genotyped as TcV. Maternal parasite loads and absorbance values by the lysate ELISA were significantly higher for transmitters compared with non-transmitters. Among seropositive mothers, 65.3% had positive results by the TSSA II/V/VI peptide ELISA. No significant difference in reactivity to TSSA II/V/VI was observed for transmitters compared with non-transmitters (79.3% vs 60.7%, respectively). CONCLUSIONS: Our findings reinforce the difficulty in obtaining sufficient sample numbers and parasite DNA to investigate the interaction between parasite genetics and the risk of congenital transmission and argue for the inclusion of DTU-specific serotyping in prospective studies.
BACKGROUND: This study identified Trypanosoma cruzi discrete typing units (DTUs) in maternal and infant specimens collected from two hospitals in Bolivia, using conventional genotyping and DTU-specific serotyping. METHODS: Specimens from 142 mothers were used, including 24 seronegative and 118 seropositive individuals; 29 women transmitted T. cruzi to their infants. Maternal and infant parasite loads were determined by quantitative real-time PCR. Maternal sera were tested with an in-house parasite lysate ELISA and serotyped by a lineage-specific peptide ELISA, targeting the trypomastigote small surface antigen (TSSA). Trypanosoma cruzi genotypes in infected infants were determined by a triple PCR-RFLP assay. RESULTS: All infant specimens were genotyped as TcV. Maternal parasite loads and absorbance values by the lysate ELISA were significantly higher for transmitters compared with non-transmitters. Among seropositive mothers, 65.3% had positive results by the TSSA II/V/VI peptide ELISA. No significant difference in reactivity to TSSA II/V/VI was observed for transmitters compared with non-transmitters (79.3% vs 60.7%, respectively). CONCLUSIONS: Our findings reinforce the difficulty in obtaining sufficient sample numbers and parasite DNA to investigate the interaction between parasite genetics and the risk of congenital transmission and argue for the inclusion of DTU-specific serotyping in prospective studies.
Authors: Pierre Buekens; María Luisa Cafferata; Jackeline Alger; Fernando Althabe; José M Belizán; Norma Bustamante; Yves Carlier; Alvaro Ciganda; Jaime H Del Cid; Eric Dumonteil; Rubí Gamboa-León; Jorge A García; Luz Gibbons; Olga Graiff; Jesús Gurubel Maldonado; Claudia Herrera; Elizabeth Howard; Laura Susana Lara; Benjamín López; María Luisa Matute; María Jesús Ramírez-Sierra; María Cecilia Robles; Sergio Sosa-Estani; Carine Truyens; Christian Valladares; Dawn M Wesson; Concepción Zúniga Journal: Am J Trop Med Hyg Date: 2017-11-30 Impact factor: 2.345
Authors: Tapan Bhattacharyya; Andrew K Falconar; Alejandro O Luquetti; Jaime A Costales; Mario J Grijalva; Michael D Lewis; Louisa A Messenger; Trang T Tran; Juan-David Ramirez; Felipe Guhl; Hernan J Carrasco; Patricio Diosque; Lineth Garcia; Sergey V Litvinov; Michael A Miles Journal: PLoS Negl Trop Dis Date: 2014-05-22