Getu Abeje1, Woyneshet Gelaye2, Getaneh Alemu3. 1. Department of Biomedcal Science, Samara University, Samara, Ethiopia. 2. Department of Medical Laboratory Science, Bahir Dar University, Bahir Dar, Ethiopia. 3. Department of Medical Laboratory Science, Bahir Dar University, Bahir Dar, Ethiopia. getanehmlt@gmail.com.
Abstract
BACKGROUND: Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodium parasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. METHODS: A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. RESULTS: Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. CONCLUSION: Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.
BACKGROUND: Both capillary and venous blood samples have been interchangeably used for the diagnosis of malaria in Ethiopia. However, Plasmodiumparasites are thought to be more concentrated in capillary than in venous blood. Hence, selecting a sample source where parasites are more concentrated is indispensable approach in order to maximize the accuracy of blood film microscopy. Therefore, the present study aimed to compare the detection rate and the parasitemia level of Plasmodium species from conventional capillary and venous blood films, and buffy coat preparations. METHODS: A facility based cross-sectional study was conducted from Feburary to March 2020 among 210 febrile patients attending Hamusite health center, northwest Ethiopia. Capillary and venous blood samples were collected and buffy coat was prepared from each sample. Thin and thick blood films were prepared, stained, and examined microscopically following standard protocol. Data were analysed using Statistical Package for Social Sciences Software version 20 and Med-Calc software version 19.3. RESULTS: Capillary blood buffy coat (61/210, 29.0%) had significantly higher detection rate as compared to capillary (48/210, 22.9%) and venous (42/210, 20.0%) blood films (p < 0.001). However, no significant difference was observed between capillary and venous blood films (p = 0.070) in detecting Plasmodium species. The highest and the lowest mean asexual stage parasite counts were found in capillary blood buffy coat (4692.88) and venous blood (631.43) films, respectively showing significant variations (p < 0.001). Mean gametocyte count was also highest in capillary blood buffy coat (3958.44). As compared to capillary blood buffy coat, the sensitivity of venous blood buffy coat, capillary blood film and venous blood film were 73.8, 78.7, 68.9%, respectively. CONCLUSION: Capillary blood buffy coat samples showed the highest sensitivity in detecting and quantitating malaria parasites that its use should be promoted in clinical settings. However, conventional capillary and venous blood films could be used interchangeably.
Entities:
Keywords:
Buffy coat; Capillary blood; Detection rate; Malaria; Venous blood
Authors: Johannes Mischlinger; Paul Pitzinger; Luzia Veletzky; Mirjam Groger; Rella Zoleko-Manego; Ayola A Adegnika; Selidji T Agnandji; Bertrand Lell; Peter G Kremsner; Egbert Tannich; Ghyslain Mombo-Ngoma; Benjamin Mordmüller; Michael Ramharter Journal: J Infect Dis Date: 2018-09-08 Impact factor: 5.226
Authors: Pierre Mukadi; Philippe Gillet; Albert Lukuka; Ben Atua; Simelo Kahodi; Jean Lokombe; Jean-Jacques Muyembe; Jan Jacobs Journal: Malar J Date: 2011-10-18 Impact factor: 2.979
Authors: Maurice M Sandeu; Albert N Bayibéki; Majoline T Tchioffo; Luc Abate; Geoffrey Gimonneau; Parfait H Awono-Ambéné; Sandrine E Nsango; Diadier Diallo; Antoine Berry; Gaétan Texier; Isabelle Morlais Journal: Malar J Date: 2017-08-17 Impact factor: 2.979