Xue Fan1, Yuhan Zhou2, Xin Guo3, Mingguo Xu4,5. 1. The Department of Pediatric Cardiology, Shenzhen Children's Hospital of China Medical University, Shenzhen, 518038, China. 2. Department of Pediatric, The Fifth Affiliated Hospital (Zhuhai) of Zunyi Medical University, 519100, Zhuhai, China. 3. The Department of Pediatric, Shenzhen Children's Hospital of China Medical University, Longgang District Maternal and Children Health Care Hospital, Shenzhen, 518038, China. 4. The Department of Pediatric Cardiology, Shenzhen Children's Hospital of China Medical University, Shenzhen, 518038, China. 18938690175@163.com. 5. The Department of Pediatric, Shenzhen Children's Hospital of China Medical University, Longgang District Maternal and Children Health Care Hospital, Shenzhen, 518038, China. 18938690175@163.com.
Abstract
BACKGROUND: Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to explore the potential molecular mechanism of KD. METHODS: PBMC was collected from one healthy child and one KD patient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells. RESULTS: Twelve cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KD child were decreased, mainly immune-related T cells, and natural killer T (NKT) cell were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell. CONCLUSIONS: Immune cell disorder appears in the KD patient at single cell level by scRNA-seq.
BACKGROUND:Kawasaki disease (KD) is the main cause of acquired heart disease in children and can lead to coronary artery lesions. This present study was designed to analyze the characteristics of KD peripheral blood mononuclear cells (PBMC) through single-cell RNA sequencing (scRNA-seq) and to explore the potential molecular mechanism of KD. METHODS:PBMC was collected from one healthy child and one KDpatient, and was used to single-cell RNA sequencing for cell clusters identification and differently expressed gene (DEG) determination. GO function enrichment analysis of DEG in B cell and T cells were performed to explore the most active biological function in KD immune cells. RESULTS: Twelve cell clusters can be identified in two samples. Compared with healthy child, naive CD8+ T cell, T helper cell and B cell in KDchild were decreased, mainly immune-related T cells, and natural killer T (NKT) cell were increased. Cell activation, lymphocyte activation and regulation of immune system process were 3 GO function shared by all four types of T cells and B cell. CONCLUSIONS: Immune cell disorder appears in the KDpatient at single cell level by scRNA-seq.