Boya Lei1,2,3, Zhujian Wang4, Qinmeng Shu1,2,3, Ruiping Gu1,2,3, Yanqiong Zhang1,2,3, Rui Jiang1,2,3, Qing Chang1,2,3, Min Zhou5,6,7, Gezhi Xu1,2,3. 1. Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. 2. Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China. 3. NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China. 4. Department of Laboratory Medicine, Eye and ENT Hospital of Fudan University, Shanghai, China. 5. Department of Ophthalmology, Eye and ENT Hospital of Fudan University, Shanghai, China. dr_minzhou@163.com. 6. Shanghai Key Laboratory of Visual Impairment and Restoration, Fudan University, Shanghai, China. dr_minzhou@163.com. 7. NHC Key Laboratory of Myopia (Fudan University), Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China. dr_minzhou@163.com.
Abstract
OBJECTIVES: To observe the changes of viral load in aqueous humour samples and visual outcomes in varicella zoster virus (VZV)-induced acute retinal necrosis (ARN). METHODS: Observational retrospective study. Medical records and viral load measured by real-time quantitative polymerase chain reaction (qPCR) of 20 eyes with VZV-induced ARN were reviewed. RESULTS: The mean viral load at presentation was 5.7 × 107 ± 9.7 × 107 copies/mL. An initial plateau phase for viral load lasting up to 2 weeks occurred in most eyes (18 eyes, 90%). In the following logarithmic reduction phase, the mean slope of the decline in viral load was -0.103 ± 0.029 log/day, and the expected time for half reduction of the initial viral load was 3.2 ± 1.0 days. At the end of the first 8-week's antiviral treatment, the viral load was below detection threshold in all 20 eyes (100.0%). The mean logarithm of the minimum angle of resolution (logMAR) best-corrected visual acuity (BCVA) improved from 1.1 ± 0.7 (Snellen equivalent 20/250) to 0.7 ± 0.6 (Snellen equivalent 20/100) after a follow-up of 8.6 ± 2.0 months. Thirteen of the 20 eyes (65.0%) suffered retinal detachment and underwent vitrectomy. The initial viral load was the independent predictive factor of logMAR BCVA at the last follow-up (β = 0.745, P < 0.001). CONCLUSIONS: The observation of viral load changes by qPCR was useful for better monitoring of therapeutic efficacy and deciding needed antiviral duration in VZV-induced ARN patients.
OBJECTIVES: To observe the changes of viral load in aqueous humour samples and visual outcomes in varicella zoster virus (VZV)-induced acute retinal necrosis (ARN). METHODS: Observational retrospective study. Medical records and viral load measured by real-time quantitative polymerase chain reaction (qPCR) of 20 eyes with VZV-induced ARN were reviewed. RESULTS: The mean viral load at presentation was 5.7 × 107 ± 9.7 × 107 copies/mL. An initial plateau phase for viral load lasting up to 2 weeks occurred in most eyes (18 eyes, 90%). In the following logarithmic reduction phase, the mean slope of the decline in viral load was -0.103 ± 0.029 log/day, and the expected time for half reduction of the initial viral load was 3.2 ± 1.0 days. At the end of the first 8-week's antiviral treatment, the viral load was below detection threshold in all 20 eyes (100.0%). The mean logarithm of the minimum angle of resolution (logMAR) best-corrected visual acuity (BCVA) improved from 1.1 ± 0.7 (Snellen equivalent 20/250) to 0.7 ± 0.6 (Snellen equivalent 20/100) after a follow-up of 8.6 ± 2.0 months. Thirteen of the 20 eyes (65.0%) suffered retinal detachment and underwent vitrectomy. The initial viral load was the independent predictive factor of logMAR BCVA at the last follow-up (β = 0.745, P < 0.001). CONCLUSIONS: The observation of viral load changes by qPCR was useful for better monitoring of therapeutic efficacy and deciding needed antiviral duration in VZV-induced ARN patients.
Authors: D A Palay; P Sternberg; J Davis; H Lewis; G N Holland; W F Mieler; D A Jabs; C Drews Journal: Am J Ophthalmol Date: 1991-09-15 Impact factor: 5.258
Authors: Charles M Calvo; Mohammed Ali Khan; Sonia Mehta; Sunir J Garg; James P Dunn Journal: Ocul Immunol Inflamm Date: 2016-01-30 Impact factor: 3.070