Ming-Hong Sun1,2, Lin-Lin Hu3, Chao-Ying Zhao4, Xiang Lu4, Yan-Ping Ren4, Jun-Li Wang3, Xiang-Shun Cui5, Shao-Chen Sun6. 1. Department of Animal Sciences, Chungbuk National University, Cheongju, 28644, South Korea. 2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. 3. The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, China. 4. College of Basic Medical Sciences, Zunyi Medical University, Zunyi, China. 5. Department of Animal Sciences, Chungbuk National University, Cheongju, 28644, South Korea. xscui@cbnu.ac.kr. 6. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China. sunsc@njau.edu.cn.
Abstract
BACKGROUND: Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse oocyte maturation. RESULTS: Our results showed that RalA/B expressed at all stages of oocyte maturation, and they were enriched at the spindle periphery area after meiosis resumption. The injection of RalA/B siRNAs into the oocytes significantly disturbed the polar body extrusion, indicating the essential roles of RalA/B for oocyte maturation. We observed that in the RalA/B knockdown oocytes the actin filament fluorescence intensity was significantly increased at the both cortex and cytoplasm, and the chromosomes were failed to locate near the cortex, indicating that RalA/B regulate actin dynamics for spindle migration in mouse oocytes. Moreover, we also found that the Golgi apparatus distribution at the spindle periphery was disturbed after RalA/B depletion. CONCLUSIONS: In summary, our results indicated that RalA/B affect actin dynamics for chromosome positioning and Golgi apparatus distribution in mouse oocytes.
BACKGROUND:Ral family is a member of Ras-like GTPase superfamily, which includes RalA and RalB. RalA/B play important roles in many cell biological functions, including cytoskeleton dynamics, cell division, membrane transport, gene expression and signal transduction. However, whether RalA/B involve into the mammalian oocyte meiosis is still unclear. This study aimed to explore the roles of RalA/B during mouse oocyte maturation. RESULTS: Our results showed that RalA/B expressed at all stages of oocyte maturation, and they were enriched at the spindle periphery area after meiosis resumption. The injection of RalA/B siRNAs into the oocytes significantly disturbed the polar body extrusion, indicating the essential roles of RalA/B for oocyte maturation. We observed that in the RalA/B knockdown oocytes the actin filament fluorescence intensity was significantly increased at the both cortex and cytoplasm, and the chromosomes were failed to locate near the cortex, indicating that RalA/B regulate actin dynamics for spindle migration in mouse oocytes. Moreover, we also found that the Golgi apparatus distribution at the spindle periphery was disturbed after RalA/B depletion. CONCLUSIONS: In summary, our results indicated that RalA/B affect actin dynamics for chromosome positioning and Golgi apparatus distribution in mouse oocytes.
Authors: Maria Almonacid; Adel Al Jord; Stephany El-Hayek; Alice Othmani; Fanny Coulpier; Sophie Lemoine; Kei Miyamoto; Robert Grosse; Christophe Klein; Tristan Piolot; Philippe Mailly; Raphaël Voituriez; Auguste Genovesio; Marie-Hélène Verlhac Journal: Dev Cell Date: 2019-10-10 Impact factor: 12.270
Authors: R Bryn Fenwick; Sunil Prasannan; Louise J Campbell; Daniel Nietlispach; Katrina A Evetts; Jacques Camonis; Helen R Mott; Darerca Owen Journal: Biochemistry Date: 2009-03-17 Impact factor: 3.162