Zhengyan Cao1,2, Peiyin Wu1,2, Hongmei Gao1, Ning Xia1, Ying Jiang1, Ning Tang3,4,5, Guohua Liu1, Zexiong Chen6,7,8. 1. College of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing, 402160, China. 2. College of Horticulture and Gardening, Yangtze University, Jingzhou, 434025, China. 3. College of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing, 402160, China. sabrina-0810@hotmail.com. 4. Chongqing Key Laboratory of Economic Plant Biotechnology, Chongqing, 400000, China. sabrina-0810@hotmail.com. 5. Collaborative Innovation Center of Special Plant Industry in Chongqing, Chongqing, 400000, China. sabrina-0810@hotmail.com. 6. College of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing, 402160, China. chenzexiong1979@163.com. 7. Chongqing Key Laboratory of Economic Plant Biotechnology, Chongqing, 400000, China. chenzexiong1979@163.com. 8. Collaborative Innovation Center of Special Plant Industry in Chongqing, Chongqing, 400000, China. chenzexiong1979@163.com.
Abstract
BACKGROUND: Lonicera macranthoides is an important woody plant with high medicinal values widely cultivated in southern China. WRKY, one of the largest transcription factor families, participates in plant development, senescence, and stress responses. However, a comprehensive study of the WRKY family in L. macranthoides hasn't been reported previously. OBJECTIVE: To establish an extensive overview of the WRKY family in L. macranthoides and identify senescence-responsive members of LmWRKYs. METHODS: RNA-Seq and phylogenetic analysis were employed to identify the LmWRKYs and their evolutionary relationships. Quantitative real-time (qRT-PCR) and transgenic technology was utilized to investigate the roles of LmWRKYs in response to developmental-, cold-, and ethylene-induced senescence. RESULTS: A total of 61 LmWRKY genes with a highly conserved motif WRKYGQK were identified. Phylogenetic analysis of LmWRKYs together with their orthologs from Arabidopsis classified them into three groups, with the number of 15, 39, and 7, respectively. 17 LmWRKYs were identified to be differentially expressed between young and aging leaves by RNA-Seq. Further qRT-PCR analysis showed 15 and 5 LmWRKY genes were significantly induced responding to tissue senescence in leaves and stems, respectively. What's more, five LmWRKYs, including LmWRKY4, LmWRKY5, LmWRKY6, LmWRKY11, and LmWRKY16 were dramatically upregulated under cold and ethylene treatment in both leaves and stems, indicating their involvements commonly in developmental- and stress-induced senescence. In addition, function analysis revealed LmWRKY16, a homolog of AtWRKY75, can accelerate plant senescence, as evidenced by leaf yellowing during reproductive growth in LmWRKY16-overexpressing tobaccos. CONCLUSION: The results lay the foundation for molecular characterization of LmWRKYs in plant senescence.
BACKGROUND: Lonicera macranthoides is an important woody plant with high medicinal values widely cultivated in southern China. WRKY, one of the largest transcription factor families, participates in plant development, senescence, and stress responses. However, a comprehensive study of the WRKY family in L. macranthoides hasn't been reported previously. OBJECTIVE: To establish an extensive overview of the WRKY family in L. macranthoides and identify senescence-responsive members of LmWRKYs. METHODS: RNA-Seq and phylogenetic analysis were employed to identify the LmWRKYs and their evolutionary relationships. Quantitative real-time (qRT-PCR) and transgenic technology was utilized to investigate the roles of LmWRKYs in response to developmental-, cold-, and ethylene-induced senescence. RESULTS: A total of 61 LmWRKY genes with a highly conserved motif WRKYGQK were identified. Phylogenetic analysis of LmWRKYs together with their orthologs from Arabidopsis classified them into three groups, with the number of 15, 39, and 7, respectively. 17 LmWRKYs were identified to be differentially expressed between young and aging leaves by RNA-Seq. Further qRT-PCR analysis showed 15 and 5 LmWRKY genes were significantly induced responding to tissue senescence in leaves and stems, respectively. What's more, five LmWRKYs, including LmWRKY4, LmWRKY5, LmWRKY6, LmWRKY11, and LmWRKY16 were dramatically upregulated under cold and ethylene treatment in both leaves and stems, indicating their involvements commonly in developmental- and stress-induced senescence. In addition, function analysis revealed LmWRKY16, a homolog of AtWRKY75, can accelerate plant senescence, as evidenced by leaf yellowing during reproductive growth in LmWRKY16-overexpressing tobaccos. CONCLUSION: The results lay the foundation for molecular characterization of LmWRKYs in plant senescence.
Authors: Deena L Rushton; Prateek Tripathi; Roel C Rabara; Jun Lin; Patricia Ringler; Ashley K Boken; Tanner J Langum; Lucas Smidt; Darius D Boomsma; Nicholas J Emme; Xianfeng Chen; John J Finer; Qingxi J Shen; Paul J Rushton Journal: Plant Biotechnol J Date: 2011-06-22 Impact factor: 9.803
Authors: Xuemin Wang; Emma Mace; Colleen Hunt; Alan Cruickshank; Robert Henzell; Heidi Parkes; David Jordan Journal: BMC Plant Biol Date: 2014-12-31 Impact factor: 4.215