Nicole T Cavanaugh1, Anutthaman Parthasarathy1, Narayan H Wong1, KayLee K Steiner1, Jonathan Chu1, Joseph Adjei2, André O Hudson3. 1. Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, 85 Lomb Memorial Drive, Rochester, NY, 14623, USA. 2. National Technical Institute for the Deaf, Rochester Institute of Technology, 52 Lomb Memorial Drive, Rochester, NY, 14623, USA. 3. Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, 85 Lomb Memorial Drive, Rochester, NY, 14623, USA. aohsbi@rit.edu.
Abstract
OBJECTIVE: In order to isolate and identify bacteria that produce potentially novel bactericidal/bacteriostatic compounds, two ponds on the campus of the Rochester Institute of Technology (RIT) were targeted as part of a bioprospecting effort. RESULTS: One of the unique isolates, RIT 452 was identified as Exiguobacterium sp. and subjected to whole-genome sequencing. The genome was assembled and in silico analysis was performed to predict the secondary metabolite gene clusters, which suggested the potential of Exiguobacterium RIT452 for producing antibiotic compounds. Extracts of spent growth media of RIT452 were active in disc diffusion assays performed against four reference strains, two Gram-negative (E. coli ATCC 25922 and P. aeruginosa ATCC 27853) and two Gram-positive (B. subtilis BGSC 168 and S. aureus ATCC 25923). Differential extraction and liquid chromatography was used to fractionate the extracts. Efforts to identify and elucidate the structure of the active compound(s) are still ongoing.
OBJECTIVE: In order to isolate and identify bacteria that produce potentially novel bactericidal/bacteriostatic compounds, two ponds on the campus of the Rochester Institute of Technology (RIT) were targeted as part of a bioprospecting effort. RESULTS: One of the unique isolates, RIT 452 was identified as Exiguobacterium sp. and subjected to whole-genome sequencing. The genome was assembled and in silico analysis was performed to predict the secondary metabolite gene clusters, which suggested the potential of Exiguobacterium RIT452 for producing antibiotic compounds. Extracts of spent growth media of RIT452 were active in disc diffusion assays performed against four reference strains, two Gram-negative (E. coli ATCC 25922 and P. aeruginosa ATCC 27853) and two Gram-positive (B. subtilis BGSC 168 and S. aureus ATCC 25923). Differential extraction and liquid chromatography was used to fractionate the extracts. Efforts to identify and elucidate the structure of the active compound(s) are still ongoing.
Authors: Archana Chauhan; Alice C Layton; Tatiana A Vishnivetskaya; Daniel Williams; Susan M Pfiffner; Bhanu Rekepalli; Brandon Stackhouse; Maggie C Y Lau; Tommy J Phelps; Nadia Mykytczuk; Jennifer Ronholm; Lyle Whyte; Tullis C Onstott; Gary S Sayler Journal: Genome Announc Date: 2014-11-20
Authors: José R Mediavilla; Amee Patrawalla; Liang Chen; Kalyan D Chavda; Barun Mathema; Christopher Vinnard; Lisa L Dever; Barry N Kreiswirth Journal: mBio Date: 2016-08-30 Impact factor: 7.867
Authors: Emily Reilly; Juan A Alfaro; Alexis R Borzelleri; Emma G Branco; Declan C Conklin; Emmaly S Held; Fio Z Kulee; Amelia J Kuzma; Nic Langdon; Alyssa M Lasko; Sean T Neri; Jasmine A Nichols; Temitope R Olawuyi; Eunice Park; Kadrian Rugullies; Carter D Wilkie; Laura R Krebs; Dawn Carter; André O Hudson; Crista B Wadsworth Journal: Microbiol Resour Announc Date: 2022-09-12