Neuroprotective Activities of Constituents from Phyllosticta capitalensis, an Endophyte Fungus of Loropetalum chinense var. rubrum.

One new dioxolanone derivative, guignardianone G ( 1 ) and twelve known compounds ( 2 - 13 ) were isolated from the 95% ethanol extract of the plant endophytic fungus Phyllosticta capitalensis cultured in rice medium. Among the known compounds isoaltenuene ( 3 ), brassicasterol ( 7 ), 5,6-epoxyergosterol ( 8 ), citreoanthrasteroid A ( 9 ), demethylincisterol A ( 10 ), chaxine C ( 11 ) were reported from Phyllosticta sp. for the first time. The structures of 1 was elucidated by 1D- and 2D-NMR experiments and HR-ESI-MS data analysis, and its absolute configuration was established through the comprehensive use of the methods of modified Mosher methods, calculations of ECD spectra and optical rotation values. The neuroprotective activity of compounds 1 - 9 , 11 - 13 were evaluated on PC12 cells damage induced by glutamate, and compounds 9 and 12 showed potential neuroprotective activities and compounds 9 and 12 with half effective concentration (EC 50 ) of 24.2 and 33.9 μ M, respectively.