Luis Fernando Saraiva Macedo Timmers1,2, Julia Vasconcellos Peixoto3, Rodrigo Gay Ducati4, José Fernando Ruggiero Bachega5, Leandro de Mattos Pereira6, Rafael Andrade Caceres5,7,8, Fernanda Majolo4, Guilherme Liberato da Silva9, Débora Bublitz Anton4, Odir Antônio Dellagostin10, João Antônio Pegas Henriques4,11, Léder Leal Xavier12, Márcia Inês Goettert4,11, Stefan Laufer13. 1. Graduate Program in Biotechnology, Universidade Do Vale Do Taquari - Univates, Lajeado, RS, Brazil. luis.timmers@univates.br. 2. Graduate Program in Medical Sciences, Universidade Do Vale Do Taquari - Univates, Lajeado, RS, Brazil. luis.timmers@univates.br. 3. Graduate Program in Cellular and Molecular Biology, Federal University of Rio Grande Do Sul - UFRGS, Porto Alegre, RS, Brazil. 4. Graduate Program in Biotechnology, Universidade Do Vale Do Taquari - Univates, Lajeado, RS, Brazil. 5. Department of Pharmacosciences, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, Brazil. 6. Laboratory of Molecular Microbial Ecology, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil. 7. Graduate Program in Biosciences, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, Brazil. 8. Graduate Program in Health Sciences, Federal University of Health Sciences of Porto Alegre - UFCSPA, Porto Alegre, RS, Brazil. 9. Laboratory of Acarology, Universidade Do Vale Do Taquari - Univates, TecnovatesLajeado, RS, Brazil. 10. Graduate Program in Biotechnology, Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas - UFPel, Pelotas, RS, Brazil. 11. Graduate Program in Medical Sciences, Universidade Do Vale Do Taquari - Univates, Lajeado, RS, Brazil. 12. Laboratory of Cell and Tissue Biology, Pontifical Catholic University of Rio Grande Do Sul - PUCRS, Porto Alegre, RS, Brazil. 13. Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, University of Tübingen, Tübingen, Germany. stefan.laufer@uni-tuebingen.de.
Abstract
Due to the high rate of transmissibility, Brazil became the new COVID-19 outbreak epicenter and, since then, is being monitored to understand how SARS-CoV-2 mutates and spreads. We combined genomic and structural analysis to evaluate genomes isolated from different regions of Brazil and show that the most prevalent mutations were located in the S, N, ORF3a and ORF6 genes, which are involved in different stages of viral life cycle and its interaction with the host cells. Structural analysis brought to light the positions of these mutations on protein structures, contributing towards studies of selective structure-based drug discovery and vaccine development.
Due to the high rate of transmissibility, Brazil becan class="Gene">me the new COVID-19 outbreak epicenter and, since then, is being monitored to understand how SARS-CoV-2mutates and spreads. We combined genomic and structural analysis to evaluate genomes isolated from different regions of Brazil and show that the most prevalent mutations were located in the S, N, ORF3a and ORF6 genes, which are involved in different stages of viral life cycle and its interaction with the host cells. Structural analysis brought to light the positions of these mutations on protein structures, contributing towards studies of selective structure-based drug discovery and vaccine development.
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