| Literature DB >> 34097274 |
Lok R Pokhrel1,2, Chukwudi S Ubah3, Sina Fallah4.
Abstract
In order for nanotechnology to be sustainably applied in agriculture, emphasis should be on comprehensive assessment of multiple endpoints, including biouptake and localization of engineered nanomaterials (ENMs), potential effects on food nutrient quality, oxidative stress responses, and crop yield, before ENMs are routinely applied in consumer and agronomic products. This chapter succinctly outlines a protocol for conducting nanophytotoxicity studies focusing on nanoparticle purification and characterization, arbuscular mycorrhizal fungi (AMF)/symbiont inoculation, biouptake and translocation/localization, varied endpoints of oxidative stress responses, and crop yield.Keywords: Agriculture; Arbuscular mycorrhizal fungi; Nanophytotoxicity; Oxidative stress; Sustainable nanotechnology; Tangential flow filtration
Year: 2021 PMID: 34097274 DOI: 10.1007/978-1-0716-1514-0_18
Source DB: PubMed Journal: Methods Mol Biol ISSN: 1064-3745