Literature DB >> 34096001

PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.

Tess C Leuthner1, Jessica H Hartman1, Ian T Ryde1, Joel N Meyer2.   

Abstract

Mitochondrial DNA (mtDNA) copy number is a critical component of overall mitochondrial health. In this chapter, we describe methods for simultaneous isolation of mtDNA and nuclear DNA (nucDNA), and measurement of their respective copy numbers using quantitative PCR. Methods differ depending on the species and cell type of the starting material, and availability of specific PCR reagents. We also briefly describe factors that affect mtDNA copy number and discuss caveats to its use as a biomarker.

Entities:  

Keywords:  Copy number; Mitochondrial DNA; Mitochondrial disease; Mitochondrial toxicity; QPCR; mtDNA; mtDNA depletion

Mesh：

Substances：

Year:  2021        PMID： 34096001     DOI： 10.1007/978-1-0716-1433-4_8

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  50 in total

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Review 8.  Mitochondrial biogenesis and dynamics in the developing and diseased heart.

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9.  C. elegans neurons jettison protein aggregates and mitochondria under neurotoxic stress.

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10.  Age-Related and Heteroplasmy-Related Variation in Human mtDNA Copy Number.

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