Literature DB >> 34095644

An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes.

Deepa Rajagopal1, Linhua Tian2, Shiqiu Xiong3, Lili Wang2, Jonathan Campbell3, Luisa Saraiva1, Sandrine Vessillier1.   

Abstract

Accurate determination of cellular subsets that secrete particular cytokine(s) is a significant parameter for functional characterization of an immunological response. The present study was conducted to develop a method for simultaneous measurement of intracellular cytokine positive CD4 and CD8 positive T lymphocytes in a single tube, with a no-wash protocol. We report here the development of a simplified, rapid procedure for precise enumeration of cytokine positive T lymphocytes using BD Trucount tubes. This single step protocol for accurate enumeration of cytokine positive T lymphocytes, will allow for better characterization of immune cell phenotype and function.

Entities:  

Keywords:  Flowcytometry; Intracellular cytokine staining; Reference materials; T lymphocyte enumeration; Trucount tubes

Year:  2020        PMID： 34095644      PMCID： PMC8176568          DOI： 10.1016/j.regen.2020.100032

Source DB:  PubMed          Journal:  J Immunol Regen Med        ISSN： 2468-4988


  7 in total

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Journal:  J Immunol Methods       Date:  2000-09-21       Impact factor: 2.303

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Authors:  Lili Wang; Fatima Abbasi; Olga Ornatsky; Kenneth D Cole; Martin Misakian; Adolfas K Gaigalas; Hua-Jun He; Gerald E Marti; Scott Tanner; Richard Stebbings
Journal:  Cytometry A       Date:  2012-04-26       Impact factor: 4.355

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Journal:  J Immunol Methods       Date:  2004-10-12       Impact factor: 2.303

Review 4.  The role of cytokines in T-cell memory in health and disease.

Authors:  Miro E Raeber; Yves Zurbuchen; Daniela Impellizzieri; Onur Boyman
Journal:  Immunol Rev       Date:  2018-05       Impact factor: 12.988

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Authors:  M E North; K Ivory; M Funauchi; A D Webster; A C Lane; J Farrant
Journal:  Clin Exp Immunol       Date:  1996-09       Impact factor: 4.330

6.  Quantification of cells with specific phenotypes I: determination of CD4+ cell count per microliter in reconstituted lyophilized human PBMC prelabeled with anti-CD4 FITC antibody.

Authors:  Richard Stebbings; Lili Wang; Janet Sutherland; Martin Kammel; Adolfas K Gaigalas; Manuela John; Bodo Roemer; Maren Kuhne; Rudolf J Schneider; Michael Braun; Andrea Engel; Dinesh K Dikshit; Fatima Abbasi; Gerald E Marti; Maria Paola Sassi; Laura Revel; Sook-Kyung Kim; Marc-Olivier Baradez; Tamara Lekishvili; Damian Marshall; Liam Whitby; Wang Jing; Volker Ost; Maxim Vonsky; Jörg Neukammer
Journal:  Cytometry A       Date:  2015-02-05       Impact factor: 4.355

Review 7.  Cytokines in Inflammatory Disease.

Authors:  Shinwan Kany; Jan Tilmann Vollrath; Borna Relja
Journal:  Int J Mol Sci       Date:  2019-11-28       Impact factor: 5.923
  7 in total

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