Erdal Bozkaya1, Nehir Canigur Bavbek2, Sila Cagri Isler3, Ahu Uraz3, Rahsan Ilikci Sagkan4, Baris Uzunok5, Sema Yuksel2. 1. Department of Orthodontics, Faculty of Dentistry, Gazi University, 8 Cd. 82.Sk. No 4 Emek, Ankara, Turkey. erdalbozkaya@gmail.com. 2. Department of Orthodontics, Faculty of Dentistry, Gazi University, 8 Cd. 82.Sk. No 4 Emek, Ankara, Turkey. 3. Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey. 4. Department of Medical Biology, School of Medicine, Usak University, Usak, Turkey. 5. Department of Physiology, School of Medicine, Usak University, Usak, Turkey.
Abstract
OBJECTIVES: The aim of this study was to assess the concentrations of heat shock protein 70 (HSP70) and toll-like receptor 4 (TLR4) during orthodontic tooth movement and to compare their levels with interleukin-1β (IL-1β), a well-known proinflammatory biomarker. MATERIALS AND METHODS: This study consisted of 20 patients (8 males, 12 females; mean age 14.75 ± 2.34 years) who needed maxillary premolar extraction and segmental canine distalization. Concentrations of HSP70, TLR4, and IL-1β were examined before extraction (T1), at the 1st (T2), 4th (T3), 7th (T4), 14th (T5), and 30th (T6) days of canine retraction by enzyme-linked immunosorbent assay analysis of gingival crevicular fluid samples. Statistical analyses were performed with repeated measure ANOVA and Spearman's rank correlation coefficient analysis (p < 0.05). RESULTS: HSP70 increased gradually from T1 to T6 and showed significant differences between T1-T6 and T2-T6 (T1:3.28 ± 0.92 ng/ml; T2:3.72 ± 0.66 ng/ml; T6:9.35 ± 2.45 ng/ml). The lowest TLR4 concentration was at T1, peaked at T3 and remained constant afterwards with significant differences between T1-T3, T1-T4, and T1-T6 (T1:0.71 ± 0.02 pg/ml; T3:1.04 ± 0.11 pg/ml; T4:0.95 ± 0.06 pg/ml; T6:1.00 ± 0.07 pg/ml). IL-1β increased from T1 to T6 with significant differences between T1-T4, T1-T5, and T1-T6 (T1:55.71 ± 5.48 pg/ml; T4:100.11 ± 16.92 pg/ml; T5:103.71 ± 23.19 pg/ml; T6:125.12 ± 22.04 pg/ml). The increase in HSP70 and TLR4 from T2-T3 showed a significant correlation (r = 0.598; p = 0.005). CONCLUSIONS: The increased levels of HSP70, TLR4, and IL-1β show the contribution of these mediators to the inflammatory response from the early stages of orthodontic tooth movement. CLINICAL RELEVANCE: The regulation of HSP70, TLR4, and/or IL-1β secretion during orthodontic force application could provide alterations for desired optimal tooth movement.
OBJECTIVES: The aim of this study was to assess the concentrations of heat shock protein 70 (HSP70) and toll-like receptor 4 (TLR4) during orthodontic tooth movement and to compare their levels with interleukin-1β (IL-1β), a well-known proinflammatory biomarker. MATERIALS AND METHODS: This study consisted of 20 patients (8 males, 12 females; mean age 14.75 ± 2.34 years) who needed maxillary premolar extraction and segmental canine distalization. Concentrations of HSP70, TLR4, and IL-1β were examined before extraction (T1), at the 1st (T2), 4th (T3), 7th (T4), 14th (T5), and 30th (T6) days of canine retraction by enzyme-linked immunosorbent assay analysis of gingival crevicular fluid samples. Statistical analyses were performed with repeated measure ANOVA and Spearman's rank correlation coefficient analysis (p < 0.05). RESULTS: HSP70 increased gradually from T1 to T6 and showed significant differences between T1-T6 and T2-T6 (T1:3.28 ± 0.92 ng/ml; T2:3.72 ± 0.66 ng/ml; T6:9.35 ± 2.45 ng/ml). The lowest TLR4 concentration was at T1, peaked at T3 and remained constant afterwards with significant differences between T1-T3, T1-T4, and T1-T6 (T1:0.71 ± 0.02 pg/ml; T3:1.04 ± 0.11 pg/ml; T4:0.95 ± 0.06 pg/ml; T6:1.00 ± 0.07 pg/ml). IL-1β increased from T1 to T6 with significant differences between T1-T4, T1-T5, and T1-T6 (T1:55.71 ± 5.48 pg/ml; T4:100.11 ± 16.92 pg/ml; T5:103.71 ± 23.19 pg/ml; T6:125.12 ± 22.04 pg/ml). The increase in HSP70 and TLR4 from T2-T3 showed a significant correlation (r = 0.598; p = 0.005). CONCLUSIONS: The increased levels of HSP70, TLR4, and IL-1β show the contribution of these mediators to the inflammatory response from the early stages of orthodontic tooth movement. CLINICAL RELEVANCE: The regulation of HSP70, TLR4, and/or IL-1β secretion during orthodontic force application could provide alterations for desired optimal tooth movement.
Authors: C C Teixeira; E Khoo; J Tran; I Chartres; Y Liu; L M Thant; I Khabensky; L P Gart; G Cisneros; M Alikhani Journal: J Dent Res Date: 2010-07-16 Impact factor: 6.116