Sustainable valorization of meat processing wastewater with synergetic eutectic mixture based purification of R-Phycoerythrin from porphyrium cruentium.

Wastewater effluent from meat processing industries are enriched with nutrients but remain underutilized. Therefore, an efficient method was administered by employing this resource for the cultivation of Porphyreum cruentium. The cultured biomass was exposed to a one-step disruption and selective purification method using a protic deep eutectic solvent to obtain a commercially significant pigment, R-Phycoerythrin (R-PE). Six protic deep eutectic solvents (pDES) were synthesized and their thermophysical activity determined. A synergy between microwave and cavitation (MACE-DLPME) has been achieved for the effective recovery of these membrane proteins. The addition of pDES provides the selective medium for the concentration of R-PE. Optimization of the MACE-DLPME method yields 95.9% (w/w) of R-PE. Preparative size exclusion chromatography resulted in (28 µg/g) of R-PE. Further ultra-purification by anion exchange chromatography enhances the purity fold of R-PE to 125. The resulting ultrapure fraction exhibits enhanced anti-platelet activity (1.56 mg/g ascorbic acid equivalent).