BACKGROUND: Characterizing the kinetics of the antibody response to SARS-CoV-2 is of critical importance to developing strategies that may mitigate the public health burden of COVID-19. We conducted a prospective, longitudinal analysis of COVID-19 convalescent plasma (CCP) donors at multiple time points over an 11-month period in order to determine how circulating antibody levels change over time following natural infection. METHODS: From April 2020 to February 2021, we enrolled 228 donors. At each study visit, subjects either donated plasma or had study samples drawn only. Anti-SARS-CoV-2 donor testing was performed using the VITROS® Anti-SARS-CoV-2 Total and IgG assays, and an in-house fluorescence reduction neutralization assay (FRNA). RESULTS: Anti-SARS-CoV-2 antibodies were identified in 97% of COVID-19 convalescent donors at initial presentation. In follow up analyses, of the 116 donors presenting for repeat timepoints, 91.4% of donors had detectable IgG levels up to 11 months post-symptom recovery, while 63% had detectable neutralizing titers, however, we observed that 25% of donors had neutralizing levels that dropped to an undetectable titer over time. CONCLUSION: Our data suggest that immunological memory is acquired in most individuals infected with SARS-CoV-2 and is sustained in a majority of patients for up to 11 months after recovery. Published by Oxford University Press for the Infectious Diseases Society of America 2021. This work is written by (a) US Government employee(s) and is in the public domain in the US.
BACKGROUND: Characterizing the kinetics of the antibody response to SARS-CoV-2 is of critical importance to developing strategies that may mitigate the public health burden of COVID-19. We conducted a prospective, longitudinal analysis of COVID-19 convalescent plasma (CCP) donors at multiple time points over an 11-month period in order to determine how circulating antibody levels change over time following natural infection. METHODS: From April 2020 to February 2021, we enrolled 228 donors. At each study visit, subjects either donated plasma or had study samples drawn only. Anti-SARS-CoV-2 donor testing was performed using the VITROS® Anti-SARS-CoV-2 Total and IgG assays, and an in-house fluorescence reduction neutralization assay (FRNA). RESULTS: Anti-SARS-CoV-2 antibodies were identified in 97% of COVID-19 convalescent donors at initial presentation. In follow up analyses, of the 116 donors presenting for repeat timepoints, 91.4% of donors had detectable IgG levels up to 11 months post-symptom recovery, while 63% had detectable neutralizing titers, however, we observed that 25% of donors had neutralizing levels that dropped to an undetectable titer over time. CONCLUSION: Our data suggest that immunological memory is acquired in most individuals infected with SARS-CoV-2 and is sustained in a majority of patients for up to 11 months after recovery. Published by Oxford University Press for the Infectious Diseases Society of America 2021. This work is written by (a) US Government employee(s) and is in the public domain in the US.
Authors: Zhongyan Lu; Eric D Laing; Jarina Pena DaMata; Katherine Pohida; Marana S Tso; Emily C Samuels; Nusrat J Epsi; Batsukh Dorjbal; Camille Lake; Stephanie A Richard; Ryan C Maves; David A Lindholm; Julia S Rozman; Caroline English; Nikhil Huprikar; Katrin Mende; Rhonda E Colombo; Christopher J Colombo; Christopher C Broder; Anuradha Ganesan; Charlotte A Lanteri; Brian K Agan; David Tribble; Mark P Simons; Clifton L Dalgard; Paul W Blair; Josh Chenoweth; Simon D Pollett; Andrew L Snow; Timothy H Burgess; Allison M W Malloy Journal: J Infect Dis Date: 2021-12-15 Impact factor: 7.759
Authors: Thomas W McDade; Amelia Sancilio; Richard D'Aquila; Brian Mustanski; Lauren A Vaught; Nina L Reiser; Matthew E Velez; Ryan R Hsieh; Daniel T Ryan; Rana Saber; Elizabeth M McNally; Alexis R Demonbreun Journal: Open Forum Infect Dis Date: 2022-03-02 Impact factor: 3.835
Authors: Manfred Nairz; Sabina Sahanic; Alex Pizzini; Anna Böhm; Piotr Tymoszuk; Anna-Maria Mitterstiller; Laura von Raffay; Philipp Grubwieser; Rosa Bellmann-Weiler; Sabine Koppelstätter; Andrea Schroll; David Haschka; Martina Zimmermann; Silvia Blunder; Kristina Trattnig; Helene Naschberger; Werner Klotz; Igor Theurl; Verena Petzer; Clemens Gehrer; John E Mindur; Anna Luger; Christoph Schwabl; Gerlig Widmann; Günter Weiss; Judith Löffler-Ragg; Ivan Tancevski; Thomas Sonnweber Journal: Sci Rep Date: 2022-03-07 Impact factor: 4.379
Authors: Kshitij Srivastava; Kamille A West; Valeria De Giorgi; Michael R Holbrook; Nicolai V Bovin; Stephen M Henry; Willy A Flegel Journal: Microbiol Spectr Date: 2021-12-08