| Literature DB >> 34080143 |
Ken-Ichi Wada1,2, Kazuo Hosokawa3, Yoshihiro Ito4, Mizuo Maeda3.
Abstract
Quantitative control of mitochondrial transfer is a promising approach for genetic manipulation of mitochondrial DNA (mtDNA) because it enables precise modulation of heteroplasmy. Furthermore, single mitochondrion transfer from a mtDNA mutation-accumulated cell to a mtDNA-less (ρ0) cell potentially achieves homoplasmy of mutated mtDNA. Here we describe the method for quantitative control of mitochondrial transfer including achieving single mitochondrion transfer between live single cells using a microfluidic device.Keywords: Cell fusion; Heteroplasmy; Homoplasmy; Microfluidics; Microtunnel; Mitochondrial DNA; Mitochondrial transfer
Year: 2021 PMID： 34080143 DOI： 10.1007/978-1-0716-1270-5_3
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745