Dibyajit Lahiri1, Moupriya Nag1, Bandita Dutta2, Soumik Dey1, Dipro Mukherjee1, Sanket J Joshi3, Rina Rani Ray2. 1. Department of Biotechnology, University of Engineering & Management, Kolkata, India. 2. Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, West Bengal, India. 3. Oil & Gas Research Center, Central Analytical & Applied Research Unit, Sultan Qaboos University, Seeb, Oman.
Abstract
AIMS: Determination of the ability of two bioactive compounds, namely Eugenol and Linalool, purified from leaves of Ocimum tenuiflorum for eradication of biofilm produced by Pseudomonas aeruginosa. METHODS AND RESULTS: The phyto-extract of Ocimum tenuiflorum (KT), a common ethno-botanical plant of India, was purified through HPLC, and was analyzed using UV spectroscopy and GC-MS. Eugenol and Linalool were found to be the most active amongst all phytocompounds present in phytoextract and showed a significant reduction in the viability of sessile cells of Pseudomonas aeruginosa and the minimum revival after withdrawal of phyto-challenge. They could bring about notable reduction in the protein and carbohydrate content of exopolysaccharide of biofilm . Eugenol and linalool could affect the synthesis of QS proteins like lasA and lasB as well as virulence factors such as pyocyanin, and rhamnolipids, which seriously hamper the formation of biofilm. The biofilm framework was extremely affected by the phytocompounds through the reduction of protein and carbohydrate content of EPS. Another interesting find out was that they brought about maximum inhibition to the genomic DNA and RNA content. The studies were supported by in-silico interaction between eugenol and linalool with the QS proteins. The antibiofilm efficacies of eugenol, linalool and phytoextract (KT) were further confirmed by microscopic studies with scanning electron (SEM), Atomic Force (AFM) and Fluorescence confocal (FCM) microscopic studies. CONCLUSIONS: The phytocompounds are proved to be more effective than conventional antibiotics in inhibiting the biofilm forming sessile cells and can be used as a replacement for antibiotic. SIGNIFICANCE AND IMPACT OF STUDY: Pure eugenol extracted from common basil leaves can be used as a safe substitute for common antibiotic for treatment of chronic infections caused by Pseudomonas aeruginosa. It will be cost effective, devoid of notable side effects and will not generate antibiotic resistance in host body. This article is protected by copyright. All rights reserved.
AIMS: Determination of the ability of two bioactive compounds, namely Eugenol and Linalool, purified from leaves of Ocimum tenuiflorum for eradication of biofilm produced by Pseudomonas aeruginosa. METHODS AND RESULTS: The phyto-extract of Ocimum tenuiflorum (KT), a common ethno-botanical plant of India, was purified through HPLC, and was analyzed using UV spectroscopy and GC-MS. Eugenol and Linalool were found to be the most active amongst all phytocompounds present in phytoextract and showed a significant reduction in the viability of sessile cells of Pseudomonas aeruginosa and the minimum revival after withdrawal of phyto-challenge. They could bring about notable reduction in the protein and carbohydrate content of exopolysaccharide of biofilm . Eugenol and linalool could affect the synthesis of QS proteins like lasA and lasB as well as virulence factors such as pyocyanin, and rhamnolipids, which seriously hamper the formation of biofilm. The biofilm framework was extremely affected by the phytocompounds through the reduction of protein and carbohydrate content of EPS. Another interesting find out was that they brought about maximum inhibition to the genomic DNA and RNA content. The studies were supported by in-silico interaction between eugenol and linalool with the QS proteins. The antibiofilm efficacies of eugenol, linalool and phytoextract (KT) were further confirmed by microscopic studies with scanning electron (SEM), Atomic Force (AFM) and Fluorescence confocal (FCM) microscopic studies. CONCLUSIONS: The phytocompounds are proved to be more effective than conventional antibiotics in inhibiting the biofilm forming sessile cells and can be used as a replacement for antibiotic. SIGNIFICANCE AND IMPACT OF STUDY: Pure eugenol extracted from common basil leaves can be used as a safe substitute for common antibiotic for treatment of chronic infections caused by Pseudomonas aeruginosa. It will be cost effective, devoid of notable side effects and will not generate antibiotic resistance in host body. This article is protected by copyright. All rights reserved.
Authors: Dominik Radzki; Marta Wilhelm-Węglarz; Katarzyna Pruska; Aida Kusiak; Iwona Ordyniec-Kwaśnica Journal: Int J Environ Res Public Health Date: 2022-03-25 Impact factor: 3.390