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Literature DB >> 3407147

The precise structure and coding capacity of mRNAs from early region 2B of human adenovirus serotype 2.

Abstract

Replication of human adenovirus (Ad) DNA requires three virus-encoded proteins that are coordinately transcribed from a single promoter at early times after infection. The mRNAs for two of these proteins, the precursor to the terminal protein (pTP) and the Ad DNA polymerase (Ad Pol), share several exons, including one encoded near Ad genome coordinate 39. The positions of the splice points of these mRNAs have been mapped by S1 nuclease mapping, by RNA sequencing, and by cDNA cloning. As a result of RNA splicing events, a short open reading frame (ORF) encoded at genome coordinate 39 is connected to the beginning of both the pTP and Ad Pol coding sequences; inclusion of this upstream ORF is essential for expression of functional pTP and Ad Pol proteins.

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Year: 1988 PMID： 3407147 DOI： 10.1016/0042-6822(88)90579-x

Source DB: PubMed Journal: Virology ISSN： 0042-6822 Impact factor: 3.616

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Cited

9 in total

1. Linker insertion mutations in the adenovirus preterminal protein that affect DNA replication activity in vivo and in vitro.

Authors: J N Fredman; S C Pettit; M S Horwitz; J A Engler
Journal: J Virol Date: 1991-09 Impact factor: 5.103

2. High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.

Authors: K Leong; W Lee; A J Berk
Journal: J Virol Date: 1990-01 Impact factor: 5.103

9. Adenovirus E4-dependent activation of the early E2 promoter is insufficient to promote the early-to-late-phase transition.

Authors: C Hemström; A Virtanen; E Bridge; G Ketner; U Pettersson
Journal: J Virol Date: 1991-03 Impact factor: 5.103

9 in total

The precise structure and coding capacity of mRNAs from early region 2B of human adenovirus serotype 2.

1. Linker insertion mutations in the adenovirus preterminal protein that affect DNA replication activity in vivo and in vitro.

2. High-level transcription from the adenovirus major late promoter requires downstream binding sites for late-phase-specific factors.

3. Mutations of the precursor to the terminal protein of adenovirus serotypes 2 and 5.

4. Construction and preliminary characterization of a library of "lethal" preterminal protein mutant adenoviruses.

5. Mutations in two cysteine-histidine-rich clusters in adenovirus type 2 DNA polymerase affect DNA binding.

6. Adenovirus type 5 precursor terminal protein-expressing 293 and HeLa cell lines.

7. Characterization of bovine adenovirus type 3 early region 2B.

8. Analysis of the adenovirus type 5 terminal protein precursor and DNA polymerase by linker insertion mutagenesis.

9. Adenovirus E4-dependent activation of the early E2 promoter is insufficient to promote the early-to-late-phase transition.