BACKGROUND: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirus nucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh. METHODS: Viral RNA was evaluated in swab specimens from 115 COVID-19 patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virus E-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19 patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA. RESULTS: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9-23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27-30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsid IgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations. CONCLUSIONS: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19 patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
BACKGROUND: The assessment of antibody responses to severe acute respiratory syndrome coronavirus-2 is potentially confounded by exposures to flaviviruses. The aims of the present research were to determine whether anti-dengue antibodies affect the viral load and the detection of anti-coronavirusnucleocapsid (N)-protein antibodies in coronavirus infectious disease 2019 (COVID-19) in Bangladesh. METHODS: Viral RNA was evaluated in swab specimens from 115 COVID-19patients by real-time reverse transcription polymerase chain reaction (rT-PCR). The anti-N-protein antibodies, anti-dengue virusE-protein antibodies and the dengue non-structural protein-1 were determined in serum from 115 COVID-19patients, 30 acute dengue fever pre-COVID-19 pandemic and nine normal controls by ELISA. RESULTS: The concentrations of viral RNA in the nasopharyngeal; Ct median (95% CI); 22 (21.9-23.3) was significantly higher than viral RNA concentrations in oropharyngeal swabs; and 29 (27-30.5) p < 0.0001. Viral RNA concentrations were not correlated with-dengue IgG levels. The anti-nucleocapsid antibodies were IgA 27% positive and IgG 35% positive at days 1 to 8 post-onset of COVID-19 symptoms versus IgA 0% and IgG 0% in dengue patients, p < 0.0001. The levels of anti- nucleocapsidIgA or IgG versus the levels of anti-dengue IgM or IgG revealed no significant correlations. CONCLUSIONS: Viral RNA and anti-nucleocapsid antibodies were detected in COVID-19patients from dengue-endemic regions of Bangladesh, independently of the dengue IgG levels.
Authors: Mohammad Jahid Hasan; Tamanna Tabassum; Mohiuddin Sharif; Mohammad Abdullah Saeed Khan; Akhi Roy Bipasha; Ariful Basher; Mohammad Rafiqul Islam; Mohammad Robed Amin; David Gozal Journal: Trans R Soc Trop Med Hyg Date: 2021-07-01 Impact factor: 2.184
Authors: Zahra Rikhtegaran Tehrani; Saman Saadat; Ebtehal Saleh; Xin Ouyang; Niel Constantine; Anthony L DeVico; Anthony D Harris; George K Lewis; Shyam Kottilil; Mohammad M Sajadi Journal: PLoS One Date: 2020-11-02 Impact factor: 3.240
Authors: Michele Spinicci; Alessandro Bartoloni; Antonia Mantella; Lorenzo Zammarchi; Gian Maria Rossolini; Alberto Antonelli Journal: Mem Inst Oswaldo Cruz Date: 2020-08-14 Impact factor: 2.743
Authors: James R Byrnes; Xin X Zhou; Irene Lui; Susanna K Elledge; Jeff E Glasgow; Shion A Lim; Rita P Loudermilk; Charles Y Chiu; Taia T Wang; Michael R Wilson; Kevin K Leung; James A Wells Journal: mSphere Date: 2020-09-16 Impact factor: 4.389