Development, processing and characterization of Polycaprolactone/Nano-Hydroxyapatite/Chitin-Nano-Whisker nanocomposite filaments for additive manufacturing of bone tissue scaffolds.

This paper focuses on utilizing the Fused Deposition Modeling (FDM) to manufacture Polycaprolactone/Nano-Hydroxyapatite/Chitin-Nano-Whisker nanocomposite scaffolds and their subsequent characterization for biomedical applications. FDM nanocomposite filaments were manufactured in multiple nanocomposite formulations of Polycaprolactone/Nano-Hydroxyapatite (nHA), Polycaprolactone/Chitin-Nano-Whisker (CNW), and Polycaprolactone/nHA/CNW using a green method. The FDM processing conditions were optimized using Taguchi orthogonal array method. The mechanical, biodegradation, and biocompatibility properties of the bone tissue scaffolds were assessed. A preosteoblast mouse bone cell line was used for cell proliferation and attachment assays. The results indicated that CNW content in the filaments slightly increases the mechanical properties of the 3D printed parts, and the nanocomposite with 3% CNW content exhibited significant improvement in the cell proliferation and attachment properties of the scaffolds. The nHA content considerably improved the mechanical properties of the scaffolds. The nHA and CNW nanofillers increased the biodegradation rate of PCL. In general, considering all types of responses, a green manufactured nanocomposite of PCL/nHA/CNW can significantly increase the biological and mechanical properties of the 3D printed products for bone tissue scaffolds.