Literature DB >> 34060035

Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes.

Gisela Beutner1, George A Porter2.   

Abstract

Native electrophoresis is a powerful tool to analyze the mitochondrial electron transport chain complexes (Cx) I-V and their assembly into supercomplexes. Valuable information regarding the composition and bioenergetic regulation in physiological and pathological conditions can be obtained. This chapter compares different types of native electrophoresis to analyze mitochondrial supercomplexes.

Keywords:  Mitochondrial supercomplexes; Native electrophoresis (blue native, colorless native, clear native, hybrid)

Year:  2021        PMID： 34060035     DOI： 10.1007/978-1-0716-1266-8_7

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  High resolution clear native electrophoresis for in-gel functional assays and fluorescence studies of membrane protein complexes.

Authors:  Ilka Wittig; Michael Karas; Hermann Schägger
Journal:  Mol Cell Proteomics       Date:  2007-04-09       Impact factor: 5.911

Review 2.  Native electrophoretic techniques to identify protein-protein interactions.

Authors:  Ilka Wittig; Hermann Schägger
Journal:  Proteomics       Date:  2009-12       Impact factor: 3.984

3.  Mass estimation of native proteins by blue native electrophoresis: principles and practical hints.

Authors:  Ilka Wittig; Tobias Beckhaus; Zibiernisha Wumaier; Michael Karas; Hermann Schägger
Journal:  Mol Cell Proteomics       Date:  2010-02-20       Impact factor: 5.911

4.  Separation by blue native and colorless native polyacrylamide gel electrophoresis of the oxidative phosphorylation complexes of yeast mitochondria solubilized by different detergents: specific staining of the different complexes.

Authors:  X Grandier-Vazeille; M Guérin
Journal:  Anal Biochem       Date:  1996-11-15       Impact factor: 3.365

5.  Functional assays in high-resolution clear native gels to quantify mitochondrial complexes in human biopsies and cell lines.

Authors:  Ilka Wittig; Rosalba Carrozzo; Filippo M Santorelli; Hermann Schägger
Journal:  Electrophoresis       Date:  2007-11       Impact factor: 3.535

6.  Hybrid gel electrophoresis using skin fibroblasts to aid in diagnosing mitochondrial disease.

Authors:  Christopher Newell; Aneal Khan; David Sinasac; John Shoffner; Marisa W Friederich; Johan L K Van Hove; Stacey Hume; Jane Shearer; Iveta Sosova
Journal:  Neurol Genet       Date:  2019-05-01

7.  Cyclophilin D regulates the dynamic assembly of mitochondrial ATP synthase into synthasomes.

Authors:  Gisela Beutner; Ryan E Alanzalon; George A Porter
Journal:  Sci Rep       Date:  2017-11-03       Impact factor: 4.379
  7 in total
  1 in total

1.  How many molecules of mitochondrial complex I are in a cell?

Authors:  Fariha Ansari; Belem Yoval; Zoya Niatsetskaya; Vadim Ten; Ilka Wittig; Alexander Galkin
Journal:  Anal Biochem       Date:  2022-03-05       Impact factor: 3.191
  1 in total

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