Literature DB >> 34057719

Multicolor Immunofluorescence Staining of Paraffin-Embedded Human Bone Marrow Sections.

Francesca M Panvini1, Simone Pacini2, Stefano Mazzoni3, Simón Méndez-Ferrer4,5,6.   

Abstract

Immunofluorescence is an indispensable method for the identification, localization and study of the expression of target antigens in formalin-fixed, paraffin-embedded (FFPE) tissue sections of human bone marrow. However, the procedure shows technical limitations because of the chemical and physical treatments required for sample processing before imaging. Here we describe a revisited protocol to obtain high-resolution images of human bone marrow trephine biopsies, improving the antigen-antibody recognition and preserving the morphology and the architecture of the bone marrow microenvironment.

Entities:  

Keywords:  Antigen retrieval; FFPE; Fixative; Immunofluorescence; Microwave heating

Mesh：

Substances：

Year:  2021        PMID： 34057719     DOI： 10.1007/978-1-0716-1425-9_10

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  10 in total

1.  Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy.

Authors:  Tomoiku Takaku; Daniela Malide; Jichun Chen; Rodrigo T Calado; Sachiko Kajigaya; Neal S Young
Journal:  Blood       Date:  2010-07-20       Impact factor: 22.113

Review 2.  Imaging methods used to study mouse and human HSC niches: Current and emerging technologies.

Authors:  Gavin Tjin; Eugenia Flores-Figueroa; Delfim Duarte; Lenny Straszkowski; Mark Scott; Reema A Khorshed; Louise E Purton; Cristina Lo Celso
Journal:  Bone       Date:  2018-04-25       Impact factor: 4.398

Review 3.  Haematopoietic stem cell activity and interactions with the niche.

Authors:  Sandra Pinho; Paul S Frenette
Journal:  Nat Rev Mol Cell Biol       Date:  2019-05       Impact factor: 94.444

4.  Increased presence of capillaries next to remodeling sites in adult human cancellous bone.

Authors:  Helene Bjoerg Kristensen; Thomas Levin Andersen; Niels Marcussen; Lars Rolighed; Jean-Marie Delaisse
Journal:  J Bone Miner Res       Date:  2013-03       Impact factor: 6.741

5.  Sample preparation for high-resolution 3D confocal imaging of mouse skeletal tissue.

Authors:  Anjali P Kusumbe; Saravana K Ramasamy; Andrea Starsichova; Ralf H Adams
Journal:  Nat Protoc       Date:  2015-10-29       Impact factor: 13.491

6.  The influence of protease digestion and duration of fixation on the immunostaining of keratins. A comparison of formalin and ethanol fixation.

Authors:  H Battifora; M Kopinski
Journal:  J Histochem Cytochem       Date:  1986-08       Impact factor: 2.479

Review 7.  Bone marrow niches in haematological malignancies.

Authors:  Simón Méndez-Ferrer; Dominique Bonnet; David P Steensma; Robert P Hasserjian; Irene M Ghobrial; John G Gribben; Michael Andreeff; Daniela S Krause
Journal:  Nat Rev Cancer       Date:  2020-02-28       Impact factor: 60.716

8.  A microwave antigen retrieval method using two heating steps for enhanced immunostaining on aldehyde-fixed paraffin-embedded tissue sections.

Authors:  Ling Gu; Jing Cong; Jie Zhang; Ying-Ying Tian; Xiao-Yue Zhai
Journal:  Histochem Cell Biol       Date:  2016-03-22       Impact factor: 4.304

Review 9.  The evolving view of the hematopoietic stem cell niche.

Authors:  Isabel Beerman; Tiago C Luis; Sofie Singbrant; Cristina Lo Celso; Simon Méndez-Ferrer
Journal:  Exp Hematol       Date:  2017-02-09       Impact factor: 3.084

10.  Multiple immunofluorescence labelling of formalin-fixed paraffin-embedded (FFPE) tissue.

Authors:  David Robertson; Kay Savage; Jorge S Reis-Filho; Clare M Isacke
Journal:  BMC Cell Biol       Date:  2008-03-19       Impact factor: 4.241
  10 in total

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