| Literature DB >> 34050469 |
Tadanobu Takahashi1, Yuuki Kurebayashi2, Tadamune Otsubo3, Kiyoshi Ikeda3, Akira Minami2, Takashi Suzuki2.
Abstract
Visualization of virus-infected cells is usually performed by immunostaining with an antiviral antibody. On the other hand, we established an easy method for fluorescence (FL) imaging of cells infected with influenza A and B viruses and some paramyxoviruses without the need for cell fixation and an antiviral antibody. These viruses and the cells they have infected express the viral surface enzyme "neuraminidase" or "hemagglutinin-neuraminidase" that shows sialidase activity. Sialidase activity is fluorescently visualized by using a sialidase fluorogenic probe developed in our previous study. The probe enables histochemical FL imaging of the virus-infected cells and is applicable to virus isolation and detection of an influenza virus resistant to antiinfluenza drugs of sialidase inhibitors.Entities:
Keywords: Drug resistance; Fluorescence imaging; Imaging probe; Infected cells; Influenza virus; Neuraminidase; Paramyxovirus; Sialidase; Virus isolation
Year: 2021 PMID： 34050469 DOI： 10.1007/978-1-0716-1258-3_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745