Literature DB >> 34043192

Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells.

Kyohei Ito1, Lejia Xu1, Genta Ito2, Taisuke Tomita3,4.   

Abstract

Recent studies revealed that leucine-rich repeat kinase 2 (LRRK2) phosphorylates several Rab proteins under physiological conditions. Mutations linked with familial Parkinson's disease cause an abnormal increase in the Rab phosphorylation, which has not been elucidated in an in vitro kinase assays where artificial peptide substrates are often used. Here, we provide protocols for detecting the LRRK2 activity in tissues and cultured cells using Rab phosphorylation as a readout.

Entities:  

Keywords:  Leucine-rich repeat kinase 2; Parkinson disease; Phos-tag; Phosphorylation; Rab

Year:  2021        PMID： 34043192     DOI： 10.1007/978-1-0716-1495-2_6

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Genome-wide association study identifies common variants at four loci as genetic risk factors for Parkinson's disease.

Authors:  Wataru Satake; Yuko Nakabayashi; Ikuko Mizuta; Yushi Hirota; Chiyomi Ito; Michiaki Kubo; Takahisa Kawaguchi; Tatsuhiko Tsunoda; Masahiko Watanabe; Atsushi Takeda; Hiroyuki Tomiyama; Kenji Nakashima; Kazuko Hasegawa; Fumiya Obata; Takeo Yoshikawa; Hideshi Kawakami; Saburo Sakoda; Mitsutoshi Yamamoto; Nobutaka Hattori; Miho Murata; Yusuke Nakamura; Tatsushi Toda
Journal:  Nat Genet       Date:  2009-11-15       Impact factor: 38.330

2.  Mutations in LRRK2 cause autosomal-dominant parkinsonism with pleomorphic pathology.

Authors:  Alexander Zimprich; Saskia Biskup; Petra Leitner; Peter Lichtner; Matthew Farrer; Sarah Lincoln; Jennifer Kachergus; Mary Hulihan; Ryan J Uitti; Donald B Calne; A Jon Stoessl; Ronald F Pfeiffer; Nadja Patenge; Iria Carballo Carbajal; Peter Vieregge; Friedrich Asmus; Bertram Müller-Myhsok; Dennis W Dickson; Thomas Meitinger; Tim M Strom; Zbigniew K Wszolek; Thomas Gasser
Journal:  Neuron       Date:  2004-11-18       Impact factor: 17.173

3.  Phos-tag analysis of Rab10 phosphorylation by LRRK2: a powerful assay for assessing kinase function and inhibitors.

Authors:  Genta Ito; Kristina Katsemonova; Francesca Tonelli; Pawel Lis; Marco A S Baptista; Natalia Shpiro; Graham Duddy; Steve Wilson; Philip Wing-Lok Ho; Shu-Leong Ho; Alastair D Reith; Dario R Alessi
Journal:  Biochem J       Date:  2016-07-29       Impact factor: 3.857

4.  Genome-wide association study reveals genetic risk underlying Parkinson's disease.

Authors:  Javier Simón-Sánchez; Claudia Schulte; Jose M Bras; Manu Sharma; J Raphael Gibbs; Daniela Berg; Coro Paisan-Ruiz; Peter Lichtner; Sonja W Scholz; Dena G Hernandez; Rejko Krüger; Monica Federoff; Christine Klein; Alison Goate; Joel Perlmutter; Michael Bonin; Michael A Nalls; Thomas Illig; Christian Gieger; Henry Houlden; Michael Steffens; Michael S Okun; Brad A Racette; Mark R Cookson; Kelly D Foote; Hubert H Fernandez; Bryan J Traynor; Stefan Schreiber; Sampath Arepalli; Ryan Zonozi; Katrina Gwinn; Marcel van der Brug; Grisel Lopez; Stephen J Chanock; Arthur Schatzkin; Yikyung Park; Albert Hollenbeck; Jianjun Gao; Xuemei Huang; Nick W Wood; Delia Lorenz; Günther Deuschl; Honglei Chen; Olaf Riess; John A Hardy; Andrew B Singleton; Thomas Gasser
Journal:  Nat Genet       Date:  2009-11-15       Impact factor: 38.330

5.  Phosphoproteomics reveals that Parkinson's disease kinase LRRK2 regulates a subset of Rab GTPases.

Authors:  Martin Steger; Francesca Tonelli; Genta Ito; Paul Davies; Matthias Trost; Melanie Vetter; Stefanie Wachter; Esben Lorentzen; Graham Duddy; Stephen Wilson; Marco As Baptista; Brian K Fiske; Matthew J Fell; John A Morrow; Alastair D Reith; Dario R Alessi; Matthias Mann
Journal:  Elife       Date:  2016-01-29       Impact factor: 8.140

6.  Cloning of the gene containing mutations that cause PARK8-linked Parkinson's disease.

Authors:  Coro Paisán-Ruíz; Shushant Jain; E Whitney Evans; William P Gilks; Javier Simón; Marcel van der Brug; Adolfo López de Munain; Silvia Aparicio; Angel Martínez Gil; Naheed Khan; Janel Johnson; Javier Ruiz Martinez; David Nicholl; Itxaso Martí Carrera; Amets Saénz Pena; Rohan de Silva; Andrew Lees; José Félix Martí-Massó; Jordi Pérez-Tur; Nick W Wood; Andrew B Singleton
Journal:  Neuron       Date:  2004-11-18       Impact factor: 17.173
  6 in total

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