Deicy Muñoz-Sánchez1, Jessica Triviño-Valencia1, Fabiana Lora-Suarez1, Jorge Enrique Gómez-Marín2. 1. Molecular Parasitology Group (GEPAMOL, for the Term in Spanish), Biomedical Research Center, Faculty of Health Sciences, Universidad del Quindío, Armenia, Colombia. 2. Molecular Parasitology Group (GEPAMOL, for the Term in Spanish), Biomedical Research Center, Faculty of Health Sciences, Universidad del Quindío, Armenia, Colombia. gepamol2@uniquindio.edu.co.
Abstract
PURPOSE: To stablish if Blastocystis subtypes influences gastrointestinal symptoms. METHODS: Case-control study. We obtained sequencing for Blastocysts subtyping from 13 patients with gastrointestinal symptoms (diarrhea or abdominal pain) and 12 from individuals without symptoms. RESULTS: 12 sequences were from Subtype 2 and one from Subtype 3 in symptomatic individuals and nine samples were from Subtype 1, one from Subtype 2, and two from Subtype 3 in asymptomatic individuals. The prevalence of subtype 2 in symptomatic individuals was vastly different compared to the frequency in asymptomatic individuals (84.6% vs. 16.6%; OR 27.5 95% CI 3.2-233; Fisher exact test p = 0.0010201335). After in vitro culture, 22 isolates were obtained. Significant differences were observed for the 12 isolates from Subtype 2 that get a smaller number of total cells with dominant growth of vacuolar forms, compared with Subtypes 1 and 3, after eight days of culture. CONCLUSION: Our results suggest that gastrointestinal symptoms in Colombian individuals with Blastocystis infection depend on the infecting subtype with peculiar phenotypic characteristics in in vitro culture.
PURPOSE: To stablish if Blastocystis subtypes influences gastrointestinal symptoms. METHODS: Case-control study. We obtained sequencing for Blastocysts subtyping from 13 patients with gastrointestinal symptoms (diarrhea or abdominal pain) and 12 from individuals without symptoms. RESULTS: 12 sequences were from Subtype 2 and one from Subtype 3 in symptomatic individuals and nine samples were from Subtype 1, one from Subtype 2, and two from Subtype 3 in asymptomatic individuals. The prevalence of subtype 2 in symptomatic individuals was vastly different compared to the frequency in asymptomatic individuals (84.6% vs. 16.6%; OR 27.5 95% CI 3.2-233; Fisher exact test p = 0.0010201335). After in vitro culture, 22 isolates were obtained. Significant differences were observed for the 12 isolates from Subtype 2 that get a smaller number of total cells with dominant growth of vacuolar forms, compared with Subtypes 1 and 3, after eight days of culture. CONCLUSION: Our results suggest that gastrointestinal symptoms in Colombian individuals with Blastocystis infection depend on the infecting subtype with peculiar phenotypic characteristics in in vitro culture.
Authors: Juan David Ramírez; Carolina Flórez; Mario Olivera; María Consuelo Bernal; Julio Cesar Giraldo Journal: PLoS One Date: 2017-02-21 Impact factor: 3.240