Forensic investigations using DNA analysis have been grown rapidly. Samples retrieved from crime scene may be exposed to different conditions before proceeding. This study aimed to evaluate the effect of different grades of temperature and burn on DNA extraction and typing. METHODS: Seven mL of blood and four mL of semen were collected from each volunteer. Effects of temperature grades (100 °C, 50 °C, 37 °C, 4 °C, -20 °C, and burn) on blood and seminal stain were tested. RESULTS: Bloodstains exposed to temperature grades 100 °C, 50 °C, 37 °C, 4 °C, and -20 °C can be identified using preliminary test while burnt blood stain cannot. Seminal stains exposed to temperature grades 37 °C, 4 °C, and -20 °C can be identified by Florence test while those exposed to 100 °C, 50 °C, and burn cannot. Blood and seminal stains exposed to temperature grades 100 °C, 50 °C, and burn show marked reduction in DNA concentration while maximum DNA conc could be recovered from stains exposed to temperature grade temperature. Both blood and seminal DNA was affected only in case of burn without significant difference between THO1 and Amelogenin primers. CONCLUSION: High environmental temperature affect the quantity of extracted DNA from different stains but less effect on the quality of extracted DNA. Burn affects both preliminary test, DNA quantity, and quality in stains.
Forensic investigations using DNA analysis have been grown rapidly. Samples retrieved from crime scene may be exposed to different conditions before proceeding. This study aimed to evaluate the effect of different grades of temperature and burn on DNA extraction and typing. METHODS: Seven mL of blood and four mL of semen were collected from each volunteer. Effects of temperature grades (100 °C, 50 °C, 37 °C, 4 °C, -20 °C, and burn) on blood and seminal stain were tested. RESULTS: Bloodstains exposed to temperature grades 100 °C, 50 °C, 37 °C, 4 °C, and -20 °C can be identified using preliminary test while burnt blood stain cannot. Seminal stains exposed to temperature grades 37 °C, 4 °C, and -20 °C can be identified by Florence test while those exposed to 100 °C, 50 °C, and burn cannot. Blood and seminal stains exposed to temperature grades 100 °C, 50 °C, and burn show marked reduction in DNA concentration while maximum DNA conc could be recovered from stains exposed to temperature grade temperature. Both blood and seminal DNA was affected only in case of burn without significant difference between THO1 and Amelogenin primers. CONCLUSION: High environmental temperature affect the quantity of extracted DNA from different stains but less effect on the quality of extracted DNA. Burn affects both preliminary test, DNA quantity, and quality in stains.
Authors: Yolanda Schaerli; Robert C Wootton; Tom Robinson; Viktor Stein; Christopher Dunsby; Mark A A Neil; Paul M W French; Andrew J Demello; Chris Abell; Florian Hollfelder Journal: Anal Chem Date: 2009-01-01 Impact factor: 6.986
Authors: Daniel G Gibson; John I Glass; Carole Lartigue; Vladimir N Noskov; Ray-Yuan Chuang; Mikkel A Algire; Gwynedd A Benders; Michael G Montague; Li Ma; Monzia M Moodie; Chuck Merryman; Sanjay Vashee; Radha Krishnakumar; Nacyra Assad-Garcia; Cynthia Andrews-Pfannkoch; Evgeniya A Denisova; Lei Young; Zhi-Qing Qi; Thomas H Segall-Shapiro; Christopher H Calvey; Prashanth P Parmar; Clyde A Hutchison; Hamilton O Smith; J Craig Venter Journal: Science Date: 2010-05-20 Impact factor: 47.728