Songtao Zhang1, Qiujie Yan2, Song Wei3, Xiaobo Feng4, Miaomiao Xue2, Lina Liu2, Jili Cui2, Yuanyuan Zhang5. 1. Department of Thyroid Head and Neck Surgery, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China. 2. Department of General Dentistry, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, China. 3. Department of Pathology, Affiliated Tumor Hospital of Zhengzhou University, Zhengzhou City, Henan Province, China. 4. Department of Dentistry and Endodontics, Anyang Stomatological Hospital, Anyang City, China. 5. Department of General Dentistry, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, China. Electronic address: Zhyy143@126.com.
Abstract
OBJECTIVES: This study aimed to evaluate the expression of chemokine-like factor superfamily 6 (CMTM6) and programmed cell death 1 (PD-1)/programmed death ligand 1 (PD-L1) in oral squamous cell carcinoma (OSCC) and to further explore its clinical significance in OSCC. STUDY DESIGN: Samples of 44 OSCC and paracancerous tissues were investigated. We estimated the expression of the 3 proteins by immunohistochemistry and further detected mRNA expression by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Immunohistochemistry results demonstrated that the positive expression of CMTM6 and PD-1/PD-L1 in OSCC tissues was significantly higher than that in paracancerous tissues. Statistical significance was found between the 2 groups (all P < .05). Moreover, PD-L1 expression was related to OSCC clinical stage and lymph node metastasis (P < .05). The qRT-PCR results confirmed that the relative expression of CMTM6 and PD-1/PD-L1 mRNA in OSCC tissues was significantly higher than that in paracancerous tissues (all P < .05), and Spearman rank correlation showed that there was a significant relationship between mRNA and protein expression (all P < .05). CONCLUSIONS: CMTM6 and PD-1/PD-L1 were upregulated in OSCC, and CMTM6 may play a synergistic role with PD-1/PD-L1 in the immune pathway. Therefore, we believe that CMTM6 and PD-1/PD-L1 will become checkpoints for immunotherapy of OSCC.
OBJECTIVES: This study aimed to evaluate the expression of chemokine-like factor superfamily 6 (CMTM6) and programmed cell death 1 (PD-1)/programmed death ligand 1 (PD-L1) in oral squamous cell carcinoma (OSCC) and to further explore its clinical significance in OSCC. STUDY DESIGN: Samples of 44 OSCC and paracancerous tissues were investigated. We estimated the expression of the 3 proteins by immunohistochemistry and further detected mRNA expression by quantitative real-time polymerase chain reaction (qRT-PCR). RESULTS: Immunohistochemistry results demonstrated that the positive expression of CMTM6 and PD-1/PD-L1 in OSCC tissues was significantly higher than that in paracancerous tissues. Statistical significance was found between the 2 groups (all P < .05). Moreover, PD-L1 expression was related to OSCC clinical stage and lymph node metastasis (P < .05). The qRT-PCR results confirmed that the relative expression of CMTM6 and PD-1/PD-L1 mRNA in OSCC tissues was significantly higher than that in paracancerous tissues (all P < .05), and Spearman rank correlation showed that there was a significant relationship between mRNA and protein expression (all P < .05). CONCLUSIONS: CMTM6 and PD-1/PD-L1 were upregulated in OSCC, and CMTM6 may play a synergistic role with PD-1/PD-L1 in the immune pathway. Therefore, we believe that CMTM6 and PD-1/PD-L1 will become checkpoints for immunotherapy of OSCC.
Authors: Ling-Ling Fu; Ming Yan; Min-Xian Ma; Yi Luo; Min Shao; Martin Gosau; Reinhard E Friedrich; Tobias Vollkommer; Hong-Chao Feng; Ralf Smeets Journal: Front Immunol Date: 2022-07-01 Impact factor: 8.786
Authors: Manuel Weber; Rainer Lutz; Manuel Olmos; Jacek Glajzer; Christoph Baran; Christopher-Philipp Nobis; Tobias Möst; Markus Eckstein; Marco Kesting; Jutta Ries Journal: Cancers (Basel) Date: 2022-04-02 Impact factor: 6.639